Reactivos e instrumentos para inmunología, biología celular y biología molecular.

Alkaline Phosphatase (ALP) Activity Assay Kit

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Referencia E-BC-K091-M-96T

embalaje : 96T

Contact local distributor :

Teléfono : +1 850 650 7790

Marca : Elabscience

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Contact local distributor :

Teléfono : +1 850 650 7790

Detection Principle	Alkaline phosphatase decompose benzene disodium phosphate to produce free phenol and phosphoric acid. Phenol react with 4-aminopyrline in alkaline solution and oxidizes with potassium ferricyanide to form red quinone derivative. The enzyme activity can be calculated indirectly by measuring the OD value.
Synonyms	ALP
Sample Type	Serum,Plasma,Tissue,Cell
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (500-530 nm, optimum wavelength: 520 nm)
Research Area	Liver And Renal Function
Other Reagents Required	Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.13 King unit/100 mL
Detection Range	0.13-50 King unit/100 mL
Precision	inter-assay CV: 8.5% \| intra-assay CV: 5.1%
Assay Time	30 min

Cat.No.	Product Name	Sizes
E-BC-K094-M	Acid Phosphatase (ACP) Activity Assay Kit	500Assays , 96T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human plasma	1
Human urine	1
Rat serum	1
Cells culture supernatant	1
10% Mouse kidney tissue homogenate	30-50
10% Mouse liver tissue homogenate	1
10% Mouse brain tissue homogenate	1
HepG2 cells	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.