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> Anti-CYB5R1 antibody (1-305)

Anti-CYB5R1 antibody (1-305)

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Referencia NB-22-72075-100

embalaje : 100uL

Marca : Neo Biotech

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Teléfono : +1 850 650 7790

  • Immunohistochemistry analysis of CYB5R1 in paraffin-embedded Rat ovary using CYB5R1 Rabbit polyclonal antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CYB5R1 in paraffin-embedded Human esophageal using CYB5R1 Rabbit polyclonal antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using CYB5R1 Rabbit polyclonal antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

General Info

Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CYB5R1 (1-305) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol: CYB5R1
Gene ID: 51706
Uniprot ID: NB5R1_HUMAN
Immunogen Region: 1-305
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-305 of human CYB5R1 (NP_057327.2).
Immunogen Sequence: MGIQTSPVLLASLGVGLVTL LGLAVGSYLVRRSRRPQVTL LDPNEKYLLRLLDKTTVSHN TKRFRFALPTAHHTLGLPVG KHIYLSTRIDGSLVIRPYTP VTSDEDQGYVDLVIKVYLKG VHPKFPEGGKMSQYLDSLKV GDVVEFRGPSGLLTYTGKGH FNIQPNKKSPPEPRVAKKLG MIAGGTGITPMLQLIRAILK VPEDPTQCFLLFANQTEKDI ILREDLEELQARYPNRFKL

Description

Tissue Specificity Widely expressed.
Function NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.
Protein Name Nadh-Cytochrome B5 Reductase 1
B5r.1
Humb5r2
Nad(Ph -Quinone Oxidoreductase Type 3 Polypeptide A2
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-1237044
Cellular Localisation Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Nadh-Cytochrome B5 Reductase 1 antibody
Anti-B5r.1 antibody
Anti-Humb5r2 antibody
Anti-Nad(Ph -Quinone Oxidoreductase Type 3 Polypeptide A2 antibody
Anti-CYB5R1 antibody
Anti-NQO3A2 antibody
Anti-UNQ3049 antibody
Anti-PRO9865 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance