Goat Polyclonal

Application / Dilution
ELISA1:2000
ICC1:1000
IHC1:1000
WB1:5000

Size 100 μl

Species Reactivity Ms

An array of chromogenic, fluorogenic and chemiluminescent substrates are available for use with HRP conjugated secondary antibodies HRP is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or the release of light as a byproduct of the reaction. HRP functions optimally at a near-neutral pH and can be inhibited by cyanides, sulfides and azides. Antibody-HRP conjugates are superior to antibody-AP conjugates with respect to the specific activities of both the enzyme and antibody. In addition, its high turnover rate, good stability, low cost and wide availability of substrates makes HRP the enzyme of choice for most applications. HRP can be used for chemiluminescent readout in western blot, colorimetric readout in ELISA, and precipitation reactions useful in immunocytochemistry and immunohistochemistry.

References

Mattson, D.L. & Bellehumeur, T.G. (1996). Anal. Biochem. 240:306.

Western blot analysis of human A549 (lane 1), MDA-MB-231 (lane 2), and MeWo (lane 3) cell lysates, as well as human breast (lane 4), lung (lane 5), skin (lane 6), and brain (lane 7) tissue lysates. The blot was probed with mouse monoclonal IgG2a isotype anti-CD44 (CM0241) at 1:2000, then anti-Mouse IgG2a specific:HRP goat polyclonal at 1:5000.



The HRP-conjugated goat polyclonal secondary reagent reacts with the Fc portion of mouse IgG2a, but does not detect other mouse IgG subclasses, IgM, or Fab portion of mouse immunoglobulins. The secondary reagent has minimal cross-reactivity with bovine, human, and rabbit immunoglobulins.

Goat polyclonal antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, and 1 mg/ml BSA. Store at –20°C. Stable for 1 year. Note: Use of sodium azide as preservative substantially inhibits the enzyme activity of HRP.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody has been pre-adsorbed with various immunoglobulins from non-mouse species before affinity-purification using mouse IgG2a coupled to agarose beads. Purified donkey polyclonal antibody was conjugated to horseradish peroxidase (HRP). This secondary reagent can be used to detect mouse IgG2a immunoglobulins in various antibody applications that utilize HRP readouts, such as western blot, ELISA, immunocytochemistry, and immunohistochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: