Anti-S protein of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human)
Referencia AS204386
embalaje : 100ul
Marca : Agrisera
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Anti-S protein of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human)
Product no: AS20 4386
AS20 4386 | Clonality: Recombinant Monoclonal | Host: Mouse | Reactivity: S protein Human SARS-CoV-2/ 2019-nCoV
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| Sub class: | Mouse scFv fusion with human IgG1 Fc |
| Immunogen: | Recombinant Human Novel Coronavirus Spike glycoprotein (S) (16-685aa), UniProt: P0DTC2 Antigen sequence has highest homology to Spike protein S1. |
| Host: | Mouse |
| Clonality: | Monoclonal recombinant |
| Purity: | Immunoglobulin purified on Protein A in 10 mM PBS, pH 7.4, 50 % glycerol, 0.03% Proclin 300. |
| Format: | Liquid |
| Quantity: | 100 µl |
| Storage: | Store at -20°C or -80°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Tested applications: | Colloidlal Gold Immunochromatography, ELISA (ELISA) |
| Recommended dilution: | 1: 500 - 1: 40 000 (Colloidal Gold Immunochromatography), 1: 10 000 - 1: 50 000 (ELISA) |
| Expected | apparent MW: | 141 kDa |
| Confirmed reactivity: | Human Novel SARS-CoV-2/ 2019-nCoV S protein |
| Not reactive in: | No confirmed exceptions from predicted reactivity are currently known |
Application example Binding Activity of SARS-CoV-2-S Antibody measured by its binding ability in a functional ELISA. Wells were coated with Recombinant Human Novel Coronavirus Spike glycoprotein(S) (AS20 4389) at 2 μg/ml. EC50 of SARS-CoV-2-S antibodies is 36.79 - 48.87 ng/ml. |
| Additional information (application): | Recombinant anti-SARS-CoV-2 spike Mouse ScFv is expressed in 293 cells (HEK293) with a human IgG1 Fc tag on C-terminal. Therefore, anti-human IgG1 Fcγ (gamma chain), secondary antibody has to be used: AS20 4390 |
| Background: | SARS-CoV-2/ 2019-nCoV S protein of Novel Coronavirus (human) its role is to attache the virion to the cell membrane by interacting with host receptor (ACE2) and initiating the infection. Alternative names: S, S1, S1-RBD, Spike glycoprotein. |