Aspartate Aminotransferase (AST/GOT) BioAssay™ Kit

Aspartate aminotransferase (AST), also known as Glutamate-oxaloacetate transaminase (GOT) is a transaminase (EC 2.6.1.1) similar to the more liver specific alanine transaminase (ALT). Although commonly included clinically as part of a diagnostic liver function test, AST has a broader clinical utility since it may also be elevated in diseases affecting other organs, such as the heart or muscles in myocardial infarction, also in acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease, musculoskeletal diseases and trauma. It catalyzes the reaction: Aspartate + ⍺-Ketoglutarate ⇌ Oxaloacetate + Glutamate Diagnostically, it is almost always measured in units/liter (U/L).||This Aspartate Aminotransferase (AST/GOT) BioAssay™ Kit can measure AST/GOT activity any kind of animal serum (plasma), tissue, culture cells and cell culture supernatant.||Detection Range:|1.1-72.3IU/L||Sensitivity:|1.1IU/L||Precision:|Intra-Assay %CV: <10%|Inter-Assay %CV: <10%||Detection Principle:|AST/GOT enables alpha-ketoglutaric acid and aspartic acid to displace amino and keto groups to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine(DNPH) to form 2,4, dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.||Kit Components:|405747A: Buffer Solution, 1x500ul|405747B: Sodium Pyruvate (2mmol/L), 1x500ul|405747C: Substrate Solution, 1x5ml|405747D: Chromogenic Agent, 1x5ml|405747E: Sodium Hydroxide, 1x5ml|405747F: Microplate, 1x96 wells||Storage and Stability:|Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.