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The goal in the development of AURION R-Gent SE-EM was to create a new system with enhancement efficiency and homogeneity at least comparable to Danscher’s method, but with reduced acidity and light sensitivity. In addition, the reagents should have low viscosity for the suitability in pre-embedding immunogold labeling.
Introduction The silver enhancement reaction is a gold particle-catalyzed reduction in which silver ions are reduced to metallic silver with a photographic developing compound as the electron source. In addition in many applications a “protective colloid” is added to the enhancement solution to limit diffusion of reagents to the gold particle surface, thus providing a means for controlling particle growth.
Product Description AURION R-Gent SE-EM is a high efficiency silver enhancement reagent for electron microscopy. It intensifies the gold particles by homogeneous deposition of metallic silver on the particle surface. The resulting electron dense signal is easily detected and is compatible with heavy metal counterstaining. The reagent has extremely delayed auto-nucleation and can be used under standard laboratory light conditions. It also has low viscosity, which is especially advantageous for pre-embedding immunogold applications. The enhancement mixture has a pH of 8.1-8.2.
AURION R-Gent SE-EM has been tested intensively for the enhancement of AURION Ultra Small Immunogold reagents.
High efficiency silver enhancement Homogeneous particles For electron microscopy Light insensitive Low viscosity solutions Virtually no auto nucleation Dropping bottles, easy preparation Mix-and-use Economical use, no left-overs
AURION R-Gent SE-EM ► Order 500.033 - 30 ml ► Order 500.044 - 90 ml
AURION R-Gent SE-EM ▼ Download Safety Data Sheet ▼ Download Technical Data Sheet