Bauhinia purpurea Lectin (BPL)

This tetrameric lectin consists of 44,000D subunits and does not require divalent cations for binding. Binding appears to be highest for glycoconjugates containing galactosyl (b-1,3) N-acetylgalactosamine structures but oligosaccharides with a terminal alpha linked N-acetylgalactosamine can also bind. Although binding specificity is similar to that of peanut agglutinin, tissue staining patterns of these two lectins are distinct.||Applications: |Suitable for use in ELISA. Other applications not tested.||Recommended Dilution:|Optimal dilutions to be determined by the researcher.||Inhibiting/Eluting Sugar: |100mM lactose||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.