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> Blocking Buffer (2X) For Fluorescent Western Blotting

Blocking Buffer (2X) For Fluorescent Western Blotting

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Referencia orb348639-50ml

embalaje : 50ml

Marca : Biorbyt

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    Blocking Buffer (2X) For Fluorescent Western Blotting

    Catalog Number: orb348639

    Catalog Numberorb348639
    CategoryProteins
    DescriptionBlocking Buffer (2X) For Fluorescent Western Blotting
    Tested applicationsWB
    Concentration2X
    Dilution rangeWB: User Defined
    Form/AppearanceLiquid (sterile filtered)
    PurityBlocking buffer is specifically formulated to achieve superior reproducible Western blotting images using this system.
    ConjugationUnconjugated
    Hazard InformationNon-Toxic
    StorageStore blocking buffer at 4° C prior to opening. DO NOT FREEZE.
    Buffer/PreservativesThimerosal is added as an antimicrobial agent.. See application note.
    Alternative namesMultiplex Blocking Buffer, Fluorescent Blocking Bu
    NoteFor research use only
    Application notesThis product is a 2X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 2X concentrate with 1 parts TBS or equivalent. This buffer was meticulously prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerosal added as an antimicrobial agent.
    Expiration Date6 months from date of receipt.
    Blocking Buffer (2X) For Fluorescent Western Blotting

    Western Blot Comparison of 1X and 2X orb348637 Buffer. Lane 1: reduced human NAG1 protein 0.1 µg. Lane 2: Prestained Molecular Weight Marker 5 µL. Lane 3: non-reduced human NAG1 protein 0.1 µg. Primary Antibody: rabbit anti-NAG1 biotin conjugated antibody (p/n orb345179) at 1 µg/mL overnight at 2-8°C. Secondary Antibody: goat anti-rabbit antibody HRP conjugated (p/n orb347654) at 1:70000 for 30 min at RT. Expect: ~14 kDa for NAG1.