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> C1q, Mouse, mAb JL-1

C1q, Mouse, mAb JL-1

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Referencia HM1096-500UG

embalaje : 500ug

Marca : Hycult Biotech

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C1q, Mouse, mAb JL-1

The monoclonal antibody JL-1 recognizes the collagen-like region (CLR) of mouse C1q, a 459 kDa molecule consisting of three individual polypeptide chains.

The monoclonal antibody JL-1 is highly specific for the collagen-like region (CLR) of mouse C1q, a key component of the classical complement pathway. C1q is a large molecule (459 kDa) composed of three distinct polypeptide chains and plays a central role in immune recognition. The JL-1 antibody was developed by immunizing C1q-deficient (C1q-/-) mice (C57BL/6 strain) with purified mouse C1q, ensuring targeted immune response toward this specific region of C1q.

C1q in the Classical Complement Pathway

C1q is part of the C1 complex, which also includes C1r and C1s, forming the initial component of the classical complement pathway. Upon interaction with immune complexes, C1q undergoes a conformational shift that activates this pathway, essential for immune defense. As the recognition unit, C1q binds to the Fc region of IgG or IgM, provided they are attached to an antigen. Additionally, C1q identifies molecular patterns on pathogens and apoptotic cells, activating the pathway and facilitating phagocytosis. This function is crucial for clearing apoptotic cells, preventing the exposure of autoantigens, and reducing immune system activation.

Importance of C1q in Immune Regulation

C1q is primarily produced by macrophages, but it is also synthesized by follicular dendritic cells and other cells in the monocyte-macrophage lineage. Deficiency in C1q can lead to impaired clearance of immune complexes and apoptotic cells, contributing to autoimmune conditions such as systemic lupus erythematosus (SLE). Inherited C1q deficiency has been strongly linked to the development of SLE, as the absence of C1q disrupts normal immune regulation.

JL-1 Antibody and Its Role in Research

The monoclonal antibody JL-1 specifically targets the collagen-like region of C1q, where autoantibodies bind in both mice and humans. These anti-C1q antibodies are often present in SLE patients and contribute to renal disease, especially in the context of glomerular immune complex disease. This makes JL-1 a valuable tool in studying autoimmune conditions like SLE and the mechanisms of C1q-related immune dysfunction.

By focusing on C1q and its pivotal role in the immune system, JL-1 provides insight into immune regulation, complement activation, and the potential development of autoimmune disorders.

Application
Frozen sections, Functional studies, Immuno assays, Immuno fluorescence, Western blot

Application Notes
IHC-F: Antibody JL-1 was used to stain tissue sections which were fixed in acetone. As positive control a polyclonal anti-C1q antibody was used and as negative control an isotype matched monoclonal antibody (Ref.1).
FS: Antibody JL-1 was administered to mice resulting in depletion of circulating C1q, glomerular deposition of C1q and induction of anti-C1q autoantibodies in susceptible mice. As a negative control an isotype matched monoclonal antibody was used (Ref.1).

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Positive Control
Spleen and kidney tissue of wild-type mice (Ref.1)

Negative Control
Spleen and kidney tissue of C1q -/- mice (Ref.1)

Product type
Monoclonal antibodies
Amount
1 mg, 10 mg, 100 µg, 20 µg, 5 mg, 500 µg
Formulation
Quantities >500 µg will be send without preservative and carrier free. Quantities <500 µg will be send with preservative and carrier. exact formulation can be found on the datasheet.
Immunogen
Purified mouse C1q
Isotype
Mouse IgG2b
Species
Mouse
Cross reactivity
Human – Yes, Rat – Yes
Alias
Complement component C1q, Complement C1q subcomponent subunit A
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product.
Disease
Nephrology, Neurological disorders
CoA-TDS
HM1096-20UG
96 kb
HM1096-100UG
105 kb
HM1096-500UG
104 kb
HM1096-1MG
104 kb
HM1096-5MG
105 kb
HM1096-10MG
105 kb
Safety Data Sheet
SDS - HM1096-20UG
51 kb
SDS - HM1096-100UG
51 kb
SDS - HM1096-500UG
50 kb
SDS - HM1096-1MG
50 kb
SDS - HM1096-5MG
50 kb
SDS - HM1096-10MG
50 kb

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