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> Calcein [1461-15-0]

Calcein [1461-15-0]

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Calcein is a fluorescent dye and self-quenching probe, used as an indicator of lipid vesicle leakage, and also as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.

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Calcein Estructura química

Calcein Estructura química

No. CAS : 1461-15-0

This product is a controlled substance and not for sale in your territory.

Based on 7 publication(s) in Google Scholar

Other Forms of Calcein:

  • Calcein (tetraethyl ester) In-stock

    Calcein purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2022, 16: 3929-3946.

    Calcein and tetracycline labeling of mice femur at 8 weeks. The red arrow indicates the distance between calcein and tetracycline fluorescence labeling.
    Descripciòn

    Calcein is a fluorescent dye and self-quenching probe, used as an indicator of lipid vesicle leakage, and also as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.

    In Vitro

    Calcein accumulates intracellularly in coelomocytes incubated in coelomic fluid or ISO-EDTA. Coelomocytes incubated in CF show an increase in the calcein fluorescence intensity compared to the control.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Calcein Related Antibodies
    Peso molecular

    622.53

    Fòrmula

    C30H26N2O13
    No. CAS

    1461-15-0
    Appearance

    Solid

    Color

    Yellow to orange

    Emission (Em)

    515
    Excitation (Ex)

    489
    SMILES

    O=C1OC2(C3=C(OC4=C2C=C(CN(CC(O)=O)CC(O)=O)C(O)=C4)C=C(O)C(CN(CC(O)=O)CC(O)=O)=C3)C5=C1C=CC=C5
    Envío

    Room temperature in continental US; may vary elsewhere.
    Almacenamiento

    RT, stored under nitrogen

    In solvent -80°C 2 years
    -20°C 1 year
    Solvente y solubilidad
    In Vitro: 

    1M NaOH : 50 mg/mL (80.32 mM; Need ultrasonic)

    H2O : 5.65 mg/mL (9.08 mM; ultrasonic and warming and adjust pH to 8 with 1 M NaOH and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.6063 mL 8.0317 mL 16.0635 mL
    5 mM 0.3213 mL 1.6063 mL 3.2127 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Calculadora de molaridad

    • Calculadora de dilución

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  2% NaHCO3 in ddH2O

      Solubility: 20 mg/mL (32.13 mM); Clear solution; Need ultrasonic and adjust pH to 6 with NaHCO3

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Pureza y Documentación

    SDS (393 KB)

    COA (254 KB) IR (137 KB)

    Instrucciones de manejo (2659 KB)
    Dyeing Example
    • Description: Calcein is a fluorescent probe with yellow green fluorescence, it can be used to determine labile iron pool and measure the Fe(III)-binding affinity with ligands.
      Method: For cell staining.
      1. Cells (106/mL) are loaded by incubation with 20 µM Fe(II) for 2 h at 37°C.
      2. Cells are collected by centrifugation (1000 rpm; 5 min) and incubated with Calcein (0.5 µM; 15 min; 37°C) in a D’hanks buffer (pH 7.4).
      3. Wash cells for twice by D’hanks buffer (pH 7.4).
      4. The fluorescence is monitored by both flow cytometry (BD FACS Caliber) and a confocal laser scanning microscope (Leica TCS SP).
    • Description: Calcein is a fluorescent probe with yellow green fluorescence, it can be used in capillary-like tube formation assay.
      Method: For cell staining.
      1. Cells are seeded at a density of 5×104 cells/well into Matrigel coated 96-well plates and treated with conditioned medium for 5.5 h under normoxia or hypoxia.
      2. Stain cells with Calcein (0.5 h).
      3. Tubes forming intact networks are captured by Lion Heart (Biotec) and quantified by counting the number of tubes.
    • Description: Calcein is a fluorescent probe with yellow green fluorescence, it can be used in tube formation assay.
      Method: For cell staining.
      1. Suspend cultured cells in conditioned medium and seeded into Matrigel coated 96-well plates at a density of 5×104 cells/well for 6 h under normoxia or hypoxia.
      2. Stain cells with Calcein (10 min).
      3. Tubes forming intact networks are captured by Lion Heart (Biotek) and quantified by the tube length and number of branch points using Image J.
    Referencias

    • [1]. Marques-Santos LF, et al. ABCB1 and ABCC1-like transporters in immune system cells from sea urchins Echinometra lucunter and Echinus esculentus and oysters Crassostrea gasar and Crassostrea gigas. Fish Shellfish Immunol. 2017 Sep 5;70:195-203.  [Content Brief]

    Ensayo celular
    [1]

    Coelomocytes suspensions (ISO and CF) are separately incubated with calcein-AM (excitation and emission wavelength: 496 nm and 516 nm, respectively) at a final concentration of 200 nM, during 30 min, at 26°C. Calcein-AM is a nonfluorescent ABC transporter substrate whose intracellular accumulation is inversely proportional to ABC transporter activity. Intracellular esterases convert calcein-AM into the fluorescent dye calcein, which is not an ABC transporter substrate, thereby accumulating the dye inside the cell. Therefore, a high fluorescence signal indicates low ABC transporter activity whereas a low fluorescence signal indicates high activity. The fluorescence of samples is measured by flow cytometer. The experiment is repeated six times in duplicates.

    MCE no ha confirmado la precisión de estos métodos independientemente. Son solo para referencia.
    Referencias

    • [1]. Marques-Santos LF, et al. ABCB1 and ABCC1-like transporters in immune system cells from sea urchins Echinometra lucunter and Echinus esculentus and oysters Crassostrea gasar and Crassostrea gigas. Fish Shellfish Immunol. 2017 Sep 5;70:195-203.  [Content Brief]

    • [1]. Marques-Santos LF, et al. ABCB1 and ABCC1-like transporters in immune system cells from sea urchins Echinometra lucunter and Echinus esculentus and oysters Crassostrea gasar and Crassostrea gigas. Fish Shellfish Immunol. 2017 Sep 5;70:195-203.

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / 1M NaOH 1 mM 1.6063 mL 8.0317 mL 16.0635 mL 40.1587 mL
    5 mM 0.3213 mL 1.6063 mL 3.2127 mL 8.0317 mL
    1M NaOH 10 mM 0.1606 mL 0.8032 mL 1.6063 mL 4.0159 mL
    15 mM 0.1071 mL 0.5354 mL 1.0709 mL 2.6772 mL
    20 mM 0.0803 mL 0.4016 mL 0.8032 mL 2.0079 mL
    25 mM 0.0643 mL 0.3213 mL 0.6425 mL 1.6063 mL
    30 mM 0.0535 mL 0.2677 mL 0.5354 mL 1.3386 mL
    40 mM 0.0402 mL 0.2008 mL 0.4016 mL 1.0040 mL
    50 mM 0.0321 mL 0.1606 mL 0.3213 mL 0.8032 mL
    60 mM 0.0268 mL 0.1339 mL 0.2677 mL 0.6693 mL
    80 mM 0.0201 mL 0.1004 mL 0.2008 mL 0.5020 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Calcein Related Classifications

    • Others
    • Others
    • Fluorescent Dye
    Help & FAQs

    Keywords:

    Calcein1461-15-0FluorexonFluorescent DyeInhibitorinhibitorinhibit

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