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Cathepsin B Activity Fluorometric Assay Kit
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Proteases/Proteasome Kit
Cathepsin B Activity Fluorometric Assay Kit
Home
Kits
Proteases/Proteasome Kit
Cathepsin Kit
Cathepsin B Activity Fluorometric Assay Kit
Cathepsin B Activity Fluorometric Assay Kit
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Background
Apoptosis is often mediated by the traditional caspase-mediated cleavage cascade. In addition, alternative proteolytic enzymes such as the lysosomal cathepsin proteases can also initiate or propagate proapoptotic signals. Cathepsins are lysosomal proteases that play an important role in mammalian cellular turnover such as bone resorption. Cathepsins are often used as sensitive markers in a variety of toxicological investigations. Cathepsin-B is an lysosomal cysteine protease belonging to the peptidase C1 family.
The Cathepsin B Activity Fluorometric Assay Kit provides a sensitive, simple and convenient way for detection of cathepsin B activity based on fluorometric method. The assay utilizes the preferred cathepsin-B substrate sequence RR labeled with AFC (amino-4-trifluoromethyl coumarin). While cleavage of the synthetic substrate RR-AFC by cathepsin-B in cell lysates or other samples, free AFC emits a yellow-green fluorescence (λmax = 505 nm) that can be easily quantified using a fluorecence microtiter plate reader or a fluorometer.
Product Citation
1. Gong Z, Zhao S, et al. "Curcumin alleviates DSS-induced colitis via inhibiting NLRP3 inflammsome activation and IL-1β production." Mol Immunol. 2018 Dec;104:11-19. PMID:30396035
2. Shengnan Zhao, Zizhen Gong, et al. "Deoxycholic acid-Mediated Sphingosine-1-Phosphate Receptor 2 Signaling Exacerbates DSS-induced Colitis through Promoting Cathepsin B Release."
Chemical Properties
Storage
Store at -20°C.
Shipping Condition
Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips
We do not recommend long-term storage for the solution, please use it up soon.