CD11b (Cell Surface Glycoprotein MAC1 Alpha Subunit, Complement Component Receptor 3 Alpha, CR3A, ITGAM, MAC1A, MO1A, Neutrophil Adherence Receptor) (FITC)

Referencia C2262-36M-100ug

embalaje : 100ug

Marca : US Biological

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C2262-36M CD11b (Cell Surface Glycoprotein MAC1 Alpha Subunit, Complement Component Receptor 3 Alpha, CR3A, ITGAM, MAC1A, MO1A, Neutrophil Adherence Receptor) (FITC)

Clone Type
Polyclonal
Host
mouse
Source
bovine
Isotype
IgG2b
Grade
Affinity Purified
Applications
FC
Crossreactivity
Bo Gt Sh
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD11b is also known as CD11b/CD18, Mac-1, alphaMbeta2 integrin, Cr3, MO-1, C3bi Receptor). CD11b is a marcophage-granulocyte specific antigen. Mac-1 macrophage differentiation antigen is associated with type three complement receptor and mediates adhesion to CD54 (ICAM-1). CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and <1% of thymocytes.||Applications:|Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilutions:|Flow Cytometry: 1:10; 10ul labels 10e6 cells in 100ul.|Optimal dilutions to be determined by the researcher.||Excitation: |490nm||Emission: |520nm||Hybridoma: |NS1 myeloma cells with spleen cells from Balb/c mice.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. |Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG2b|Clone No: 10B594 (CC126)|Host: mouse|Source: bovine|Concentration: ~0.1mg/ml|Form: Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). |Purity: Purified by Protein G affinity chromatography.|Specificity: Recognizes bovine CD11b cell surface antigen. Species Crossreactivity: goat and sheep||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes bovine CD11b cell surface antigen. Species Crossreactivity: goat and sheep
References
1. Howard, C.J. and Naessens, J. (1993) Immunopathol. 39: 25-48, 2. Naessens, J. et al. (1993) Immunopathol. 39: 283-290. 3 Akesson, C.P. et al. (2008) Dev Comp Immunol. 32: 837-49. 4. Lecchi, C. et al. (2008) Vet Res. 39: 50. 5. Connelley, T. et al. (2011) Vet Res. 42: 37. 6. Kallapur, S.G. et al. (2011) Am J Physiol Lung Cell Mol Physiol. Jun 10. [Epub ahead of print] 7. Albenzio, M. and Caroprese, M. (2010) J. Dairy Research. 78: 43-48. 8. Graff, J.C. and Jutila, M.A. (2007) J Leukoc Biol. 82: 603-7. 9. Top, S. et al. (2011) J Virol. 85: 12982-94. 10. Albenzio, M. et al. (2012) J Dairy Sci. 95: 3877-87.