CD29 (Integrin beta 1) (FITC)

C2381-10B stimulates the adherance of several cell types. ||Applications: |Suitable for use in Flow Cytometry (Not Tested), Immunohistochemistry, ELISA, Immunoprecipiation and Western Blot. Other applications not tested.||Recommended Dilutions:|Flow Cytometry (Not Tested): 1ug/10e6 cells|Immunohistochemistry (Frozen): 5ug/ml|ELISA: 0.1-1ug/ml|Immunoprecipitation: 1-5ug|Optimal working dilutions to be determined by researcher.||Recommended Secondary Antibodies:|I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo|I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo|I1904-14C: IgG, Fc (HRP) Pab Gt xMo|I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo||Recommended Negative Control:|I1904-83P1: IgG2a Murine Negative Control||Hybridoma: |NS1 myeloma cells with spleen cells from Balb/c mice.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. |Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ||Note: Applications are based on unconjugated antibody.