CD3e (Early T Cell Marker)

CD3e (Early T Cell Marker)

Referencia C2254-01-100ul

embalaje : 100ul

Marca : US Biological

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C2254-01 CD3e (Early T Cell Marker)

Clone Type
Polyclonal
Host
rabbit
Source
human
Isotype
IgG
Grade
Supernatant
Applications
IHC
Crossreactivity
Ca Eq Fe Hu Mk
Shipping Temp
Blue Ice
Storage Temp
-20°C

Monoclonal antibodies clustered as CD3 recognize the constant structure of the CD3/TcR (T cell receptor) complex. This complex is present on mature T cells and during thymopoiesis. CD3/TcR is called the T cell receptor complex. This complex is composed of different structures, i.e. TcRa, TcRb, TcRg, TcRd, CD3g, CD3d, CD3e, the x-chain, and the h-chain. Less than 10% of human peripheral T cells express the g/d TcR complex. The function of a/b and g/d heterodimers is the recognition of peptide antigen bound to MHC, followed by T cell activation. Recent data suggest that CD3 chains are able to function autonomously in signal transduction. T cells play a major role in activation processes of the immune system. Detection of these cells is important during immune monitoring. In addition, a large number of leukemias are T cell-related disorders, in which antibodies recognizing CD3/TcR complex are important diagnostic tools. Differentiation between AML, T-ALL and B-ALL can be made by immunophenotyping with (early) differentiation markers. Cell surface detection using CD3 antibodies can be applied for detection of mature T-ALL, while immature T-ALL express cytoplasmic (cyCD3) but no surface CD3. CD3 antibodies are used, in characterization of various subtypes of chronic lymphoid leukemias. Examples of these chronic T cell leukemias are T-CLL (Sézary Syndrome) and the peripheral T cell lymphoma (ATLL) which co-express CD2, CD3, CD4 and CD5 antigens. ||Applications:|Suitable for use in Immunohistochemistry. Other applications have not been tested.||Recommended Dilutions:|Immunohistochemistry (FFPE): 1:150 for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min. followed by cooling at RT for 20 min.|Optimal dilutions to be determined by the researcher.||Positive Control: |Jurkat cells, Tonsil||Cellular Localization: |Cell membrane||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG|Clone No: 3N1 (SP7)|Host: rabbit|Source: human|Concentration: Not Determined |Form: Supplied as concentrated tissue culture supernatant with 15mM sodium azide.|Purity: Tissue culture supernatant|Immunogen: Synthetic 13-mer peptide corresponding to aa156-168 of the epsilon chain of human CD3 protein.|Specificity: Recognizes the intracytoplasmic portion of the human CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues. Species Crossreactivity: baboon, monkey, equine, canine and feline.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Synthetic 13-mer peptide corresponding to aa156-168 of the epsilon chain of human CD3 protein.
Form
Supplied as concentrated tissue culture supernatant with 15mM sodium azide.
Purity
Tissue culture supernatant
Specificity
Recognizes the intracytoplasmic portion of the human CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues. Species Crossreactivity: baboon, monkey, equine, canine and feline.
References
1. Spieker-Polet H, et al. Proc Natl Acad Sci. 1995 Sep 26; 92(20):9348-52. 2. Liguori MJ, et al. Hybridoma. 2001 Jun; 20(3):189-98. 3. G.Cano1,et al., 1Garcia da Orta Hospital, Almada, Portugal,2 Inst. Molec. Pathology and Immunology of Porto University, Portugal,3 Medical Faculty of Porto university, Portugal Diagn Cytopathol, 2003 Oct; 29(4): 207 -11. 4. L.K. Diaz* and N.Sneige *Department of Pathology,Northwestern University, Chicago,+ Department of Pathology, University of Texas, Huston, Adv Anat Pathol,2005; 12(1), 10-19.