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> CD95 / FAS / TNFRSF6(FAS/3588), CF700 conjugate, 0.1mg/mL

CD95 / FAS / TNFRSF6(FAS/3588), CF700 conjugate, 0.1mg/mL

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Referencia BNC003588-500

embalaje : 500uL

Marca : Biotium

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Analysis of Protein Array containing more than 19,000 full-length human proteins using CD95 Mouse Monoclonal Antibody (FAS/3588). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
Antibody number

#3588
Antibody reactivity (target)

CD95, FAS, TNFRSF6
Antibody type

Primary
Host species

Mouse
Clonality

Monoclonal
Clone

FAS/3588
Isotype

IgG2b, kappa
Molecular weight

38-50 kDa
Synonyms

ALPS1A; Apoptosis APO1 antigen 1; Apoptosis-mediating surface antigen FAS; APT1; Delta Fas; Fas AMA; Fas cell surface death receptor; FAS1; FASL receptor; FASLG receptor; FASTM; Surface antigen APO1; Tumor necrosis factor receptor superfamily member 6 (TNFRSF6)
Human gene symbol

FAS
Entrez gene ID

355
SwissProt

P25445
Unigene

244139
Immunogen

Recombinant fragment (around aa26-96) of human CD95 (FAS) protein (exact sequence is proprietary)
Verified antibody applications

IHC (FFPE) (verified)
Antibody target cellular localization

Plasma membrane
Species reactivity

Human
Positive control

MCF-7 cells (IF/FACS). Human hepatocellular or bladder carcinoma (IHC).
Shipping condition

Room temperature
Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Regulatory status

For research use only (RUO)
Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Validated in protein array

Monospecific
Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended
Product origin

Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.

Download Datasheet

Product Description

This MAb specifically recognizes CD95, also known as Fas, a transmembrane glycoprotein with a MW of 40-45 kDa, containing 8 kDa of N-glycoside-linked polysaccharide. It is a receptor for TNFSF6/FASLG, a member of the nerve growth factor receptor/tumor necrosis factor superfamily, mediating receptor-triggered apoptosis. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation, which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). CD95 antigen is expressed on the surface of various cell types, preferentially on the CD45RAlow CD45ROhigh subset of memory T lymphocytes.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the

CF® Dye Brochure

for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email

info@clinisciences.com

to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 3588, Anti-CD95|FAS|TNFRSF6 (FAS/3588) 

Antibody # prefix Conjugation Ex/Em (nm) Laser line Detection channel Dye Features
BNC04 CF®405S 404/431 405 DAPI (microscopy), AF405

CF®405S Features
BNC88 CF®488A 490/515 488 GFP, FITC

CF®488A Features
BNC68 CF®568 562/583 532, 561 RFP, TRITC

CF®568 Features
BNC94 CF®594 593/614 561 Texas Red®

CF®594 Features
BNC40 CF®640R 642/662 633-640 Cy®5

CF®640R Features
BNC47 CF®647 650/665 633-640 Cy®5

CF®647 Features
BNC74 CF®740 742/767 633-685 775/50

CF®740 Features
BNCB Biotin N/A N/A N/A
BNUB Purified N/A N/A N/A
BNUM Purified,
BSA-free		 N/A N/A N/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.