CFTR Human Gene Knockout Kit (CRISPR)
CAT#: KN216476RB
CFTR - human gene knockout kit via CRISPR, HDR mediated
Functional Cassette: GFP-puro Luciferase-Puro mBFP-Neo
HDR-mediated knockout kit validation
Product Images
Specifications
| Product Data | |
| Format | 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control |
| Donor DNA | RFP-BSD |
| Symbol | CFTR |
| Locus ID | 1080 |
| Components | KN216476G1, CFTR gRNA vector 1 in pCas-Guide CRISPR vector KN216476G2, CFTR gRNA vector 2 in pCas-Guide CRISPR vector KN216476RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette. GE100003, scramble sequence in pCas-Guide vector |
| Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
| Reference Data | |
| RefSeq | NM_000492 |
| UniProt ID | P13569 |
| Synonyms | ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1; MRP7; TNR-CFTR |
| Summary | This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. The encoded protein functions as a chloride channel, making it unique among members of this protein family, and controls ion and water secretion and absorption in epithelial tissues. Channel activation is mediated by cycles of regulatory domain phosphorylation, ATP-binding by the nucleotide-binding domains, and ATP hydrolysis. Mutations in this gene cause cystic fibrosis, the most common lethal genetic disorder in populations of Northern European descent. The most frequently occurring mutation in cystic fibrosis, DeltaF508, results in impaired folding and trafficking of the encoded protein. Multiple pseudogenes have been identified in the human genome. [provided by RefSeq, Aug 2017] |
Documents
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Resources
Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| KN216476 | CFTR - human gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN216476BN | CFTR - human gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN216476LP | CFTR - human gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN416476 | CFTR - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. | 1 657,00 EUR | |
| GA100776 | CFTR CRISPRa kit - CRISPR gene activation of human CF transmembrane conductance regulator | 1 657,00 EUR |