Complement C3c (Rhodamine)

Human Complement component 3, C3 (alternative names include acylation-stimulating protein (ASP) C3 is encoded by gene located 19p13.3-p13.2. Because C3, C4, and C5 are strikingly similar suggesting a common evolutionary origin. C3 is an acute phase reactant. Synthesis of C3, a glycoprotein, is induced during acute inflammation. The liver is the main site of synthesis, although small amounts are also produced by activated monocytes and macrophages. A single chain precursor (pro-C3) of approximately 200kD is found intracellularly; the cDNA shows that it comprises 1,663aa. This is processed by proteolytic cleavage into alpha (~115kD) and beta subunits (~75kD) which in the mature protein are linked by disulfide bonds. ||Applications: |Suitable for use in FLISA, Immunoelectrophoresis and single and double radial immunodiffusion. Other applications not tested.||Recommended Dilution:|Immunoelectrophoresis: 2ml plasma or equivalent against 120ml antiserum|Double Radial Immunodiffusion: Use a rosette arrangement with 10ml antiserum in 3mm|diameter center well and 2ml plasma samples (neat and serially diluted) in 2mm diameter|peripheral wells.|Single Radial Immunodiffusion: 1% in the gel.|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.