Gain Consistency with our Single Lot Media and Reagents

Kit consists of 500mL SF-4Z0-500 Serum-Free Medium, 8mL vial of RocketFuel™ (containing animal derived growth factors), and 10mL vial of Attachment Factor™ (extracellular matrix reagent for coating flasks). This becomes a complete medium once activated with the included RocketFuel™ supplement. This yields a chemically defined complete medium which does not require the addition of bovine serum. SF-4Z0-500 is Certified and intended for experimental application.

Available Formats

Available in one 500 mL container with 8mL and 10mL vials.

Characteristics of Cell Systems Serum-Free Medium:

Medium contains no added hormones. Medium contains no antibiotics; however, Bac-Off® (4Z0-643), is the recommended antibiotic for all Cell Systems media. Bac-Off® is used at or above the 2µg/mL MIC of aerobic Gram-positive and Gram-negative microorganisms.

Medium contains phenol red.

Addition of Bovine Serum is not necessary.

Cell Systems Serum-Free Medium is a chemically defined, complete medium which does not contain bovine-derived components. Please note that RocketFuel™ does contain bovine-derived growth factors. For a bovine-free supplement, see RocketFuel-R™. Human serum albumin and other components of human origin are specified by the formulation.

Use with Attachment Factor™

For best results, use Cell Systems Medium in conjunction with cell culture vessels coated with Attachment Factor™ extracellular matrix reagent. See the Attachment Factor™ protocol.

Medium Storage

Cell Systems Medium is shipped at ambient temperature. If the entire unit will not be used within 30 days, supplement the medium with CultureBoost™, then aliquot and freeze in smaller units which will be used within 30 days. Otherwise, immediately refrigerate the medium and supplement. Once the unit is supplemented, or any component of the medium kit is opened, the shelf life is 30 days at refrigerated (2 - 8°C) temperatures.

SF-4Z0-500 is Certified and intended for experimental application. Cell Systems media and reagents are Sterile, made with WFI and all components are cGMP and ISO Compliant.