K2104-100 assays | Cytochrome c Apoptosis Assay Kit Clinisciences

Background

Cytochrome c is located between the inner and the outer mitochondrial membranes. Once activated by the apoptosis stimulus, cytochrome c is released from mitochondria into cytosol where it couples to Apaf-1. The cytochrome c/Apaf-1 complex further activates the downstream caspases. The Cytochrome c Releasing Apoptosis Assay Kit gives an efficient way for sensing cytochrome c releasing from mitochondria into cytosol under apoptosis. The kit utilizes a special formulation of reagents to isolate enriched mitochondrial fraction from cytosol. The protocol is simple to perform without unltracentrifugations and toxic chemicals. The cytochrome c antibody provided in the kit is then used to determine the Cytochrome c releasing from mitochondria into cytosol by Western blotting.

Features & Properties

Features	Simple procedure; Takes approx. 3 hours; Fast and convenient; Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit. The procedure is simple and straightforward. No ultracentrifugation is required.
Shipping	Dry Ice
Storage Conditions	Multi

Quality Control

Storage

Multi

Overview

Kit components

Mitochondria Extraction Buffer, 5X Cytosol Extraction Buffer, DTT (1 M, 500X Protease Inhibitor Cocktail, Anti-Cytochrome c mouse mAb (0.2 mg/mL)

Cytochrome c Apoptosis Assay Kit

Referencia K2104-100 assays

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Marca : APExBIO Technology