Dextranase antibody (Peroxidase)
Referencia orb344723-100ug
embalaje : 100ug
Marca : Biorbyt
Item 1 of 2
Item 1 of 2
Dextranase antibody (Peroxidase)
Catalog Number: orb344723
Catalog Number | orb344723 |
---|---|
Category | Antibodies |
Description | Dextranase antibody (Peroxidase) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IP, WB |
Reactivity | Bacteria |
Isotype | IgG |
Immunogen | Dextranase [Penicillium species] |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:2,000 - 1:10,000, IP: User Optimized, WB: 1:500 - 1:5,000 |
Form/Appearance | Lyophilized |
Purity | Dextranase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Dextranase [Penicillium species]. Cross reactivity against Dextranase from other sources is unknown. |
Conjugation | HRP |
UniProt ID | P48845 |
NCBI | AAB47720.1 |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Alternative names | rabbit anti-Dextranase Antibody Peroxidase Conjuga |
Note | For research use only |
Application notes | Anti-Dextranase Peroxidase has been tested by ELISA and western blot and assayed against 1.0 ug of Dextranase [Penicillium species] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product. |
Expiration Date | 12 months from date of receipt. |
ELISA Results of Rabbit Anti-Dextranase Antibody Peroxidase Conjugate tested against purified Dextranase. Each well was coated in duplicate with 1.0 µg of Dextranase. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer and TMB substrate (p/n orb348651).
Western Blot of Rabbit Anti-Dextranase Antibody Peroxidase Conjugated. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: Dextranase. Primary Antibody: Rabbit Anti-Dextranase at 1:1000 overnight at 2-8°C. Secondary Antibody: none. Block: orb348637. Expect MW: ~66 kDa.