DNA Marker 50bp Ladder, 13 Fragments (50-1000bp), BioGenomics™

Referencia D3930-05-50ug

embalaje : 50ug

Marca : US Biological

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D3930-05 DNA Marker 50bp Ladder, 13 Fragments (50-1000bp), BioGenomics™

Clone Type
Polyclonal
Grade
Molecular Biology Grade
Shipping Temp
Blue Ice
Storage Temp
-20°C

DNA Marker 50bp Ladder is designed for sizing and approximate quantification of wide range double-stranded DNA on agarose and polyacrylamide gels. The ladder is composed of 13 chromato- graphically purified individual DNA fragments (in base pairs): 1000, 900, 800, 700, 600, 500, 400, 300, 250, 200, 150, 100, 50.|Note: The bright bands at 500 and 250bp serve as the reference.||Concentration:|0.5mg DNA/ml||Storage Buffer:|10mM Tris-HCl (pH 7.6), 1mM EDTA||6X Loading Dye Solution:|Supplied as a liquid in 10mM Tris-HCl, pH 7.6, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol, 60mM EDTA.||Quality Control:|Analysis of 0.5ug (1ul) of the DNA Ladder on agarose gel and 1ug (2ul)-on polyacrylamide gel by ethidium bromide staining generates 13 discrete band patterns.||Quantity of DNA in Band:|Fragment (bp) % ng/0.5ug DNA|1000 6 30|900 6 30|800 6 30 |700 6 30 |600 6 30 |500 15 75 |400 6 30 |300 6 30|250 15 75|200 7 35|150 7 35|100 7 35|50 7 35|Total 100 500||Storage and Stability:|May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Stellwagen, N.C., Biochemistry, 22: 6,183-6,193 (1983). 2. Lane, D., et al., Microbiological Reviews, 56: 509-528 (1992). 3. Stellwagen, N.C., Electrophoresis, 21: 2,327-2,334 (2000).