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> Donkey IgG (H&L) antibody

Donkey IgG (H&L) antibody

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Referencia orb347992-2mg

embalaje : 2mg

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    Donkey IgG (H&L) antibody

    Catalog Number: orb347992

    Catalog Numberorb347992
    CategoryAntibodies
    DescriptionDonkey IgG (H&L) antibody
    Species/HostGoat
    ClonalityPolyclonal
    Tested applicationsELISA, IHC, WB
    ReactivityDonkey
    IsotypeIgG
    ImmunogenDonkey IgG whole molecule
    Concentration2.0 mg/mL
    Dilution rangeELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
    Form/AppearanceLiquid (sterile filtered)
    PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Donkey IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Donkey IgG and Donkey Serum.
    ConjugationUnconjugated
    StorageStore vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesgoat anti-Donkey IgG Antibody, goat anti Donkey Ig
    NoteFor research use only
    Application notesAnti-Donkey IgG antibody has been tested by ELISA and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
    Expiration Date12 months from date of receipt.
    Donkey IgG (H&L) antibody

    ELISA Results of Goat Anti-Donkey IgG Antibody tested against purified Donkey IgG. Each well was coated in duplicate with 1.0 µg of Donkey IgG. The working dilution is 1:38200. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Donkey Anti-Goat IgG HRP conjugated (p/n orb347032), and TMB substrate (p/n orb348651).