EasyPure® EndoFree Plasmid MaxiPrep Kit
Referencia EM123-01
embalaje : 10rxns
Marca : TransGen Biotech
Contact local distributor :
Teléfono : +1 850 650 7790
ManualEasyPure® EndoFree Pro Plasmid MaxiPrep Kit uses a modified alkaline lysis method to isolate high quality plasmid DNA
from≤500 ml(LB) of bacterial culture. The solution contains indicators that can indicate whether the lysis and
neutralization are complete through the change of color, so as to ensure the quality of plasmid extraction and visualize the
operation. Plasmid purification process include special endotoxin removal solution. Endotoxin level can reach as low as
<0.1 EU/μg. The purified DNA is suitable for a variety of molecular biology applications including restriction enzyme
digestion, ligation, transformation, DNA sequencing, and transfection.
Features
• Visualized operation: solution LB (blue) indicates whether the lysis and neutralization are complete through the
change of color.
• Fast: the whole procedure can be performed in one hour.
• High purity: high-purity, endotoxin-free (<0.1EU/μg) transfection-grade plasmid DNA can be prepared.
• High yield (up to 4 mg nucleic acid load in the purification column).
| Component | EM123-01(10 rxns) |
| Resuspension Buffer (RB) | 120 ml |
| Lysis Buffer (LB, Blue) | 120 ml |
| Neutralization Buffer 1 (NB1) | 120 ml |
| Activation Buffer (AB) | 55 ml |
| Endotoxin Removal Buffer (ER) | 33 ml |
| Wash Buffer (WB) | 25 ml |
| Elution Buffer (EB) | 25 ml |
| RNase A (10 mg/ml ) | 1.2 ml |
| Maxi-Plasmid Spin Column with Collection Tube | 10 each |
| Push Filter | 10 each |
| 50 ml Collection Tube | 10 each |