EasyPure® Plasmid MiniPrep Kit
Referencia EM101-02
embalaje : 200rxns
Marca : TransGen Biotech
Contact local distributor :
Teléfono : +1 850 650 7790
ManualEasyPure® Plasmid MiniPrep Kit uses a modified alkaline lysis method followed by adsorption of DNA onto silica membrane
to isolate high-quality plasmid DNA from ≤20 ml cultures of E.coli in LB medium. DNA yield up to 40 μg. The solution
contains indicators that can indicate whether the lysis and neutralization are complete through the change of color, so as to
ensure the quality of plasmid extraction and visualize the operation. The purified plasmid DNA is suitable for a variety of
molecular biology applications, including restriction enzyme digestion, ligation, transformation and DNA sequencing.
• Simple and fast: the whole procedure can be performed in 20 minutes.
• High yield: DNA yield up to 40 μg.
Storage
at room temperature (15-25°C) in a dry place for one year.
Shipping
at room temperature.
| Component | EM101-02 (200 rxns) |
| Resuspension Buffer (RB) | 60 ml |
| Lysis Buffer (LB, Blue) | 60 ml |
| Neutralization Buffer (NB, Yellow) | 80 ml |
| Wash Buffer (WB) | 2×20 ml |
| Elution Buffer (EB) | 10 ml |
| RNase A (10 mg/ml) | 600 μl |
| Mini-Plasmid Spin Columns with Collection Tubes | 2×100 |
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