EasyScript® First-Strand cDNA Synthesis SuperMix

Referencia AE301-03

embalaje : 100rxns×20ul

Marca : TransGen Biotech

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Teléfono : +1 850 650 7790

EasyScript® First-Strand cDNA Synthesis SuperMix

Catalog Number: AE301-02

Price:Please inquire first

Specification:
50 rxns×20 µl system
100 rxns×20 µl system

Product Details

EasyScript® First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by EasyScript® RT/RI Enzyme Mix and 2×ES Reaction Mix.

• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• The product obtained from 15 minutes reaction is used for qPCR; the, product obtained from 30 minutes reaction is used for PCR.
• Anchored Oligo(dT)18 Primer is specifically designed to bind to the first base next to mRNA Poly(A)+ on the 5' end with high specificity, ensuring high efficiency and success rate of first-strand cDNA synthesis.
• Random Primer (N9) or Gene Specific Primer (GSP) can be used to synthesize the first-strand cDNA.

• cDNA up to 8 kb.

Applications
Multiple copy gene detection

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Composition


Component   AE301-02   AE301-03
EasyScript® RT/RI Enzyme Mix          50 μl        100 μl
2×ES Reaction Mix        500 μl           1 ml
Random Primer(N9) (0.1 μg/μl)          50 μl        100 μl
Anchored Oligo(dT)18 Primer (0.5 μg/μl)          50 μl        100 μl
RNase-free Water        500 μl           1 ml


References

1 Dai B, Wang F, Wang Y, et al. Targeting HDAC3 to overcome the resistance to ATRA or arsenic in acute promyelocytic leukemia through ubiquitination and degradation of PML-RARα[J]. Cell Death & Differentiation, 2023.(IF 12.07)

2 Chen H, Qian X, Chen X, et al. Cytoplasmic and nuclear Sw‐5b NLR act both independently and synergistically to confer full host defense against tospovirus infection[J]. New Phytologist, 2021.(IF 8.51)

3 Xiao W, Hu S, Zou X, et al. Lectin receptor-like kinase LecRK-VIII. 2 is a missing link in MAPK signaling-mediated yield control[J]. Plant physiology, 2021.(IF 8.34)

4 Yue X, Song J, Fang B, et al. BcNRAMP1 promotes the absorption of cadmium and manganese in Arabidopsis[J]. Chemosphere, 2021.(IF 7.08)

5 Zheng J, Lu Y, Xiao J, et al. Pan-HDAC inhibitors augment IL2-induced proliferation of NK cells via the JAK2-STAT5B signaling pathway[J]. International Immunopharmacology, 2023.(IF 5.70)


FAQ

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