EnzyChrom™ Glutathione Reductase Kit
Application
- For quantitative determination of glutathione reductase activity.
Key Features
- Fast and sensitive. Linear detection range (20 µL sample): 0.4 to 50 U/L for 20 min reaction. Detection Limit of 0.4 U/L.
- Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method
- OD412nm
Samples
- Plasma, serum, tissue and culture media etc
Species
- All
Procedure
- 30 min
Size
- 100 tests
Detection Limit
- 0.4 U/L
Shelf Life
- 6 months
More Details
- GLUTATHIONE REDUCTASE (GR) reduces oxidized glutathione (GSSG) to the reduced sulfhydryl form GSH which is an important cellular antioxidant. A high GSH/GSSG ratio is important for protection against oxidative stress. Thus, measurement of GR activity is used as indicator for oxidative stress. BioAssay Systems’ non-radioactive, colorimetric GR assay is designed to accurately measure GR activity in biological samples with a method that utilizes Ellman’s method in which DTNB reacts with the GSH generated from the reduction of GSSG by the GR in a sample to form a yellow product (TNB2-). The rate of change in the optical density, measured at 412 nm, is directly proportional to GR activity in the sampl
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KHAN, ZAFAR JAVED, et al (2019). Indian Journal of Novel Drug Delivery. Indian Journal of Novel Drug Delivery 11.1: 30-39. Assay: Glutathione reductase in rat serum.
Bardell, DA et al (2015). Measures of Redox Balance in Horses Undergoing Corrective Surgery Involving Strangulating Lesions of the Small Intestine. Equine Vet J. 47(S48): 6. Assay: Glutathione reductase in horse plasma.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost