Goat IgG ELISA Kit (Goat)
Referencia OKCA00272
embalaje : 96Wells
Marca : Aviva Systems Biology
Contact local distributor :
Teléfono : +1 850 650 7790
| Predicted Species Reactivity | Goat | ||||||||||||||||||||||||||||||||||||||||||
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| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Duration | 2.5 Hours | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | Aviva Systems Biology Goat IgG ELISA Kit (Goat) (OKCA00272) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with Goat IgG and blocked. Standards or samples are added to the plate wells along with a horseradish peroxidase (HRP) conjugated antibody specific to Goat IgG and incubated. Goat IgG immobilized in the wells competes with the liquid phase Goat IgG contained in the samples or standards for binding with the HRP-Conjugated Goat IgG Antibody. Wells are washed removing unbound Goat IgG, unbound HRP-Conjugated Goat IgG Antibody or Goat IgG /HRP-Conjugated Goat IgG Antibody complex. HRP-Conjugated Goat IgG Antibody bound to Goat IgG immobilized in the well remains. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of HRP-Conjugated Goat IgG Antibody captured in the well and inversely proportional to the amount of Goat IgG which was contained in the sample or standard. | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Range | 0.15 ug/mL - 37.5 ug/mL | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Reproducibility |
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| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||||||||||||||||||||||
| Sample Type | Serum, Plasma | ||||||||||||||||||||||||||||||||||||||||||
| Sensitivity | < 0.15 ug/mL | ||||||||||||||||||||||||||||||||||||||||||
| Specificity | This assay has high sensitivity and excellent specificity for detection of goat IgG. No significant cross-reactivity or interference between goat IgG and analogues was observed. | ||||||||||||||||||||||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive Immunoassay | ||||||||||||||||||||||||||||||||||||||||||
| Gene Symbol | Goat IgG |
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