GRK 2,3 (betaARK 1,2) (APC)

Applications:|Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.||Recommended Dilutions:|Immunoprecipitation: This antibody can be used to immunoprecipitate GRK2/3 from transfected cells. |Western Blot: 0.1-1ug/ml. Detects GRK2/3 in RIPA lysates from mouse 3T3 fibroblasts. Previous lots of this antibody detected GRK2/3 in bovine brain cytosol preparation, rat L6 myoblasts, human A431 carcinoma and human HeLa cell RIPA lysates. 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with G8972 (0.1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. ||Recommended secondary antibodies: |I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo |I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo||Positive Control Included:|F4209: Fibroblast Cell, 3T3/A31.|Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.||Storage and Stability: |May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. ||Note: Applications are based on unconjugated antibody.