GUINEA PIG COMPLEMENT (fresh frozen)
Referencia C300-0010
embalaje : 10mL
Marca : Rockland Immunochemicals
Contact local distributor :
Teléfono : +1 850 650 7790
Specifications for Guinea Pig Complement (Fresh Frozen)
Product Details
Guinea Pig Complement (Fresh Frozen) - C300-0010
Complement system, tissue macrophages, blood monocytes, protease C3-convertase, mannose-binding lectin pathway, C3, C3a, C3b, C5a, C5b, C6, C7, C8, and polymeric C9, cascade cleavage and activation events, recruit inflammatory cells, anaphylatoxin
Guinea Pig
Application Details
Cellular Assay, Purification - View References
Guinea Pig Complement (Fresh Frozen) is designed for use in a range of immunological applications, including complement fixation tests (CFT), serum radial hemolysis (SRH), and B-cell purification. It exhibits standard physiological properties such as normal pH, immunoelectrophoresis, hemoglobin, and IgG concentration, ensuring reliability across experiments. In cellular assays, the complement shows robust activity with low cytotoxic background.
Tissue Data
Complement
Mixed
Guinea Pig - Mixed
Formulation
Non-sterile
82mg/ml by Refractometry
None
None
None
Shipping & Handling
Dry Ice
Store Guinea Pig Complement at -70° C prior to opening. Aliquot contents and freeze at -70° C or below. Use aseptic technique to maintain sterility when opening product. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. COMPLEMENT IS A TEMPERATURE SENSITIVE PRODUCT. IMPROPER STORAGE WILL INACTIVATE COMPLEMENT ACTIVITY.
Expiration date is one (1) year from date of receipt.
Background
Guinea Pig Complement (Fresh Frozen) from Rockland Immunochemicals is optimized for high complement activity. Extracted and preserved in a fresh frozen state, this guinea pig serum-derived complement maximizes activity and stability, making it ideal for use in immunogenicity assays and other immunology-based applications.
References (21)
Gu H et al. (2023). Ultra-high static magnetic fields cause immunosuppression through disrupting B-cell peripheral differentiation and negatively regulating BCR signaling. MedComm (2020).
Applications
Incubation PubMed
Guan F et al. (2023). GSDMA3 deficiency reprograms cellular metabolism and modulates BCR signaling in murine B cells. iScience.
Applications
Incubation PubMed
Zhu, Y et al. (2022). Involvement of MST1/mTORC1/STAT1 activity in the regulation of B-cell receptor signalling by chemokine receptor 2. Clinical and Translational Medicine
Applications
B-Cell Purification PubMed
Luo L et al. (2022). Abelson tyrosine kinase controls BCR signalling and B-cell differentiation by promoting B-cell metabolism. Immunology.
Applications
Incubation PubMed
Yang L et al. (2021). CCL2 regulation of MST1-mTOR-STAT1 signaling axis controls BCR signaling and B-cell differentiation. Cell Death Differ.
Applications
B-Cell Purification PubMed
Li N, Jiang P, Chen A, et al. (2020). CX3CR1 positively regulates BCR signaling coupled with cell metabolism via negatively controlling actin remodeling. Cell Mol Life Sci.
Applications
Other PubMed
Jing Y. et. al. (2020). STING couples with PI3K to regulate actin reorganization during BCR activation. IMMUNOLOGY
Applications
B-Cell Purification PubMed
Yang JS et al. (2019). IgM specific to lipopolysaccharide of Vibrio cholerae is a surrogate antibody isotype responsible for serum vibriocidal activity. PLoS One.
Applications
Cellular assay PubMed
Hosseini SM et al. (2015). Transcriptome profiling of bovine inner cell mass and trophectoderm derived from in vivo generated blastocysts. BMC Dev Biol.
Applications
Inner Cell Mass (ICM) PubMed
Chen WH et al. (2014). Safety and immunogenicity of escalating dosages of a single oral administration of peru-15 pCTB, a candidate live, attenuated vaccine against enterotoxigenic Escherichia coli and Vibrio cholerae. Clin Vaccine Immunol.
Applications
Vibriocidal Assay PubMed
Zhang J et al. (2012). Development and characterization of an infectious cDNA clone of the modified live virus vaccine strain of equine arteritis virus. Clin Vaccine Immunol.
Applications
Other PubMed
Ozawa M et al. (2012). Importance of culture conditions during the morula-to-blastocyst period on capacity of inner cell-mass cells of bovine blastocysts for establishment of self-renewing pluripotent cells. Theriogenology.
Applications
Inner Cell Mass (ICM) PubMed
LaFleur RL et al. (2010). One-year duration of immunity induced by vaccination with a canine Lyme disease bacterin. Clin Vaccine Immunol.
Applications
Cellular assay PubMed
Yang JS et al. (2009). A duplex vibriocidal assay to simultaneously measure bactericidal antibody titers against Vibrio cholerae O1 Inaba and Ogawa serotypes. J Microbiol Methods.
Applications
Vibriocidal Assay PubMed
Yang JS et al. (2007). A semi-automated vibriocidal assay for improved measurement of cholera vaccine-induced immune responses. J Microbiol Methods.
Applications
Vibriocidal Assay PubMed
Whitaker-Menezes D et al. (2003). An epithelial target site in experimental graft-versus-host disease and cytokine-mediated cytotoxicity is defined by cytokeratin 15 expression. Biol Blood Marrow Transplant.
Applications
Other PubMed
Lynch JM et al. (2003). Increased protection against pneumococcal disease by mucosal administration of conjugate vaccine plus interleukin-12. Infect Immun.
Applications
Other PubMed
Jones SC et al. (2003). Post-hematopoietic cell transplantation control of graft-versus-host disease by donor CD4+ 25+ T cells to allow an effective graft-versus-leukemia response. Biol Blood Marrow Transplant.
Applications
Other PubMed
Patterson AE et al. (2001). Infusion of select leukemia-reactive TCR Vbeta+ T cells provides graft-versus-leukemia responses with minimization of graft-versus-host disease following murine hematopoietic stem cell transplantation. Biol Blood Marrow Transplant.
Applications
Other PubMed
Schnaper HW wt al. (1984). Identification and initial characterization of concanavalin A- and interferon-induced human suppressor factors: evidence for a human equivalent of murine soluble immune response suppressor (SIRS). J Immunol.
Applications
Undefined PubMed
Rittenberg MB et al. (1972). In vitro initiated secondary anti-hapten response. 3. Separable roles of hapten and carrier in immune paralysis. Immunohistochemistry.
Applications
In Vitro Plaque-forming Cell (PFC) Assay PubMed
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Certificate of Analysis Lookup
Disclaimer
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.