HaCaT
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HaCaT Cells
Essential facts about Hacat cells
Description | HaCaT cells are a pivotal model in dermatological research, offering insights into the complex mechanisms of skin biology and pathology. The spontaneously immortalized HaCaT cell line is derived from adult human epidermal cells and retains the capacity to proliferate and undergo differentiation, similar to basal keratinocytes in vivo. HaCaT cells serve as a robust platform for investigating the epidermal differentiation process and studying the epidermal differentiation markers essential for maintaining skin integrity. The susceptibility of HaCaT cells to apoptosis and their sensitivity to apoptosis-inducing agents are extensively studied, particularly in the context of cytotoxic agents like RIPL. Researchers assess these agents' cytotoxicities and the extent of cytotoxicity using HaCaT cells, utilizing techniques such as fluorescence microscopy to visualize cellular changes. Researchers have leveraged HaCaT cells to examine the effects of various agents, including antimicrobial substrates and their influence on cell viability. These cells are an excellent substrate for testing antimicrobial biomaterials and antimicrobial atelocollagen substrates, crucial for skin repair and medical applications. The HaCaT epidermal line also plays a crucial role in studying cellular senescence, cytokines, and gene expression profiles related to aging and chronic diseases. The transcriptional profiles of HaCaT cells, including the role of κB and microRNAs, provide insight into the regulatory mechanisms at the molecular level. The HaCaT keratinocyte line, with their characteristics as epidermal keratinocytes, offers a tractable system for dissecting the intricate interplay between epidermal cells and the immune system, specifically the role of keratinocytes in disease states. They enable the exploration of epigenetic modifications and their influence on the differentiation of keratinocytes, including the formation of the cornified envelope, a key feature in the skin's barrier function. In summary, HaCaT cells are an indispensable model in dermatological research, facilitating a deeper understanding of skin biology and pathology through their resemblance to basal keratinocytes and their ability to undergo cell growth and differentiation. Their application spans from studying epidermal differentiation and antimicrobial effects to exploring cellular responses such as apoptosis, making them a cornerstone in cell biology and biomedical research. |
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Organism | Human |
Tissue | Skin |
Details
Age | 62 years |
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Gender | Male |
Ethnicity | Caucasian |
Cell type | Keratinocytes with a diameter of 20-25 micrometer. |
Growth properties | Adherent |
Documentation
Citation | HaCaT (Cytion catalog number 300493) |
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Biosafety level | 1 |
Depositor | DKFZ, Heidelberg |
Genetics of the HaCaT keratinocyte cell line
Tumorigenic | No |
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Karyotype | Aneuploid (hypotetraploid) |
Handling the Hacat cell line
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | The 1:1 mixture of EDTA (stock. 0.05%) and trypsin (stock: 0.1%) must be prepared each time ahead of detaching the cells using PBS without Ca2+ and Mg2+ to provide a physiologic osmolarity. Ready-to-use mixtures of trypsin/EDTA are not recommended, as this may result in cell clumps. As an alternative, TrypLE Express (Life Technologies) instead of trypsin/EDTA can be used. The protocol of the manufacturer should be followed. |
Doubling time | The doubling time of HaCaT cells is 28 hours. |
Subculturing |
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Split ratio | A ratio of 1:5 to 1:10 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality assurance
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,x CSF1PO: 9,11 D13S317: 10,12 D16S539: 9,12 D5S818: 12 D7S820: 9,11 TH01: 9.3 TPOX: 11,12 vWA: 16,17 D3S1358: 16 D21S11: 28,30.2 D18S51: 12 Penta E: 7,12 Penta D: 11,13 D8S1179: 14 FGA: 24 D1S1656: 11,12 D2S1338: 17,25 D12S391: 18,23 D19S433: 13,14 |
HLA alleles | A*: 01.01.1900 07:01 B*: 01.01.1900 16:01, 02.01.1900 03:01 C*: 03:04:01, 15:02:01 DRB1*: 04:01:01, 15:01:01 DQA1*: 01:02:01, 03:03:01 DQB1*: 03:01:01, 06:02:01 DPB1*: 03:01:01, 04:01:01 E: 01:03:01, 01:03:02 |