Hep-2C cells
Referencia C0008008
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Origin:
Cervix carcinoma
Origin:
Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination. This line was originally thought to be derived from an epidermoid carcinoma of the larynx, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination. The cells are positive for keratin by immunoperoxidase staining.
Species:
Homo sapiens, human HeLa derivative, human cervix carcinoma Epithelial Adherent Modal no. 76 Female, Black AddexBio-formulated EMEM (C0005-01) + 10% FBS Subculture sub-confluent cultures (85%) at 1:3 to 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C. Complete growth culture medium supplemented with 7.5% (v/v) DMSO II Bacteria: Negative Yeast: Negative Mycoplasma: Negative HIV: Negative Hepatitis B: Negative HPV: Negative EBV: Negative CMV: Negative Amelogenin: X CSF1PO: 9,10 D13S317: 12,13.3 D16S539: 9,10 D5S818:11,12 D7S820: 8,12 THO1:7 TPOX: 8,12 vWA: 16,18 If use of this culture results in a scientific publication, it should be cited in the publication as: Hep-2C (AddexBio C0008008) Tissue:
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References for Hep-2C cells:
1. Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PMID: 833871