Histone H3 pT3/K4ac antibody
Referencia orb420383-50ug
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Histone H3 pT3/K4ac antibody
Catalog Number: orb420383
| Catalog Number | orb420383 |
|---|---|
| Category | Antibodies |
| Description | Histone H3 pT3/K4ac antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ChIP, DOT, IF, IHC, Multiplex Assay, WB |
| Reactivity | C. elegans, Human, Mouse |
| Isotype | IgG |
| Immunogen | Histone H3 [ac Lys4, p Thr3] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with synthetic acetylated/phosphorylated peptide surrounding Lysine 4 and Threonine 3 of human Histone H3. |
| Concentration | 0.57 mg/mL |
| Dilution range | ChIP: 2-5µg/million cells, IHC: 1:50, IF: 1:50, WB: 1:500 |
| Form/Appearance | Liquid (sterile filtered) |
| Purity | Anti-Histone H3 [ac Lys4, p Thr3] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined. |
| Conjugation | Unconjugated |
| UniProt ID | Q71DI3 |
| NCBI | NP_001005464 |
| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Alternative names | rabbit anti-Histone H3 Ac Lys4 pT3 antibody, H3.3B |
| Note | For research use only |
| Application notes | Anti-Histone H3 [ac Lys4, p Thr3] antibody is tested for Western Blot, Immunocytochemistry/Immunofluorescence, and Dot Blot. This antibody is useful in Chromatin Immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals. |
| Expiration Date | 12 months from date of receipt. |
Dot Blot of Rabbit Histone H3 [p Thr3, ac Lys4] Antibody. Lane 1: pT3. Lane 2: pT3/K4me1. Lane 3: pT3/K4me2. Lane 4: pT3/K4ac. Lane 5: pT3/RZm2s. Lane 6: K4 unmodified. Lane 7: K4me1. Lane 8: K4me2. Lane 9: K4me3. Lane 10: K4me ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [p Thr3, ac Lys4] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
Immunofluorescence Microscopy of Rabbit Anti-Histone H3 [ac Lys4, p Thr3] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [ac Lys4, p Thr3] antibody at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10000 for 45 min at RT. Localization: Histone H3 [ac Lys4, p Thr3] is nuclear and chromosomal. Staining: Histone H3 [ac Lys4, p Thr3] is expressed in green while the nuclei and alpha-tubulin were coexpressed with DAPI (blue) and Dylight 550 (red).
Western Blot of Rabbit Anti-Histone H3 [p Thr3, ac Lys4] Antibody. Lane 1: HeLa histone prep. 2. NIH-3T3 histone prep. Lane 3: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [p Thr3, ac Lys4] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.