Human HSP90 alpha antibody
Referencia AHS0704-50
embalaje : 50ul
Marca : NKMAX BIO
Contact local distributor :
Teléfono : +1 850 650 7790
ICC/IF analysis of HSP90 in HeLa cells. The cell was stained with AHS00704 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysates(20ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP90 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 1.: HeLa cell lysate
Flow cytometry (FACS)
Flow cytometry analysis of HSP90 in HepG2 cells. The cell was stained with AHS0704 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Immunohistochemistry (IHC)
Paraffin embedded sections of human urothelium were incubated with anti-human Hsp90 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.