600129 | Human IL-6 Clinisciences

Recombinant Active Protein

Cat. No.

600129

Unit

0.02 mg

Description

Cytokines of the IL6/GCSF/MGF family are glycoproteins of about 170 to 180 amino acid residues that contains four conserved cysteine residues involved in two disulphide bonds. Interleukin-6 (IL-6) is an important proinflammatory and immunoregulatory cytokine expressed by various cells. IL-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro. Recombinant Human IL-6 produced in E. coli is a single non-glycosylated polypeptide chain containing 184 amino acids with a MW of 21,000 Da.

Spec Container

Datasheet

Format

Each vial contains 20 ug protein lyophilized in 10 mM acetic acid. Reconstitute with 0.2 ml sterile 10 mM HCl for a final concentration of 0.1 mg/ml.

Alternate Names

interleukin-6; IL-6; B-cell stimulatory factor 2; BSF-2; interferon beta-2; hybridoma growth factor; CTL differentiation factor; CDF

Accession No.

P05231

21000 Da

Sequence

PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM

Source

E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Purity

Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Application Notes

Human IL-6 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of mouse 7TD1 cells is less then 0.08 ng/ml.

Storage

Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.

Images

For research use only, not for diagnostic or therapeutic procedures.

Human IL-6

Referencia 600129

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Marca : Abbiotec

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Spec Container