Human TNF-alpha
Referencia 600173
embalaje : 0.05mg
Marca : Abbiotec
Contact local distributor :
Teléfono : +1 850 650 7790
Description
TNF (Tumor necrosis factor) is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells following their stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-alpha while CD8 cells secrete little or no TNF-alpha. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, and GM-CSF. Genetic variations in TNF are associated with susceptibility to psoriasis and hepatitis B virus infection. Recombinant Human Tumor Necrosis Factor-alpha produced in E. coli is a single non-glycosylated polypeptide chain containing 158 amino acids with a MW of 17,483.77 Da.
Each vial contains 50 ug protein lyophilized in 10 mM sodium phosphate pH8.0, 20 mM NaCl. Reconstitute with 0.5 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Alternate Names
tumor necrosis; TNF- alpha; tumor necrosis factor ligand superfamily member 2; TNF-a; cachectin; tumor necrosis factor, membrane form; tumor necrosis factor, soluble form
Accession No.
MW
17484 Da
Sequence
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL
Source
E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Purity
Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Application Notes
Recombinant Tumor Necrosis Factor-alpha is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D is less than 0.05 ng/ml.
Storage
Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
For research use only, not for diagnostic or therapeutic procedures.