IgA, Heavy Chain (Biotin)

IgA is the predominant immunoglobulin class in body secretions, such as saliva, tears, bronchial secretions, nasal mucosal secretions, prostatic fluid, vaginal secretions, and mucous secretions of the small intestines. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. It is also found in small amounts in blood. Because it is resistant to degradation by enzymes, secretory IgA can survive in harsh environments such as the digestive and respiratory tracts, to provide protection against microbes that multiply in body secretions. IgA does not activate complement, and opsonises only weakly. Its heavy chains are of the type . It exists in two forms, IgA1 (90%) and IgA2 (10%): IgA1 is found in serum and made by bone marrow B cells.||Applications:|Suitable for use in Immunohistochemistry, ELISA, Immunoprecipitation and Western Blot. Other applications not tested. ||Recommended Dilutions:|Immunohistochemistry: Frozen sections|Optimal dilutions to be determined by the researcher.||Hybridoma: |NS1 myeloma cells with spleen cells from Balb/c mice. ||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.