ELISA:5-10 µg/ml. Functional Assays. Flow Cytometry: Membrane permeabilization is required. We recommend the use of Leucoperm for this purpose.
Reactivity
Bovine, Equine, Goat, Sheep
Antibody Host
Mouse
Isotype
IgG1
Clonality
Monoclonal
Immunogen
Plasmid cDNA encoding Bovine IL-10. Spleen cells from immunised Balb/c mice were fused with cells of the mouse sp2/0 myeloma cell line.
Specificity
This antibody recognizes IL-10. Clone CC320 has been shown to neutralise the activity of bovine IL-10 as measured by the inhibition of the inhibitory activity of IL-10 on IFN gamma synthesis (Buza et al. 2004).
Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 178 amino acids. There is 73% homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1.
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