IL6 Chemi-Luminescent ELISA Kit (Mouse)

Referencia OKCD03686

embalaje : 96Wells

Marca : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for IL6 Chemi-Luminescent ELISA Kit (Mouse) (OKCD03686)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationCLIA
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)82-101%98-105%89-101%92-99%
EDTA Plasma (n=5)91-99%85-92%82-98%91-103%
Heparin Plasma (n=5)90-99%88-101%84-103%98-105%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 6 (IL6). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 6 (IL6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 6 (IL6) level in the sample or standard.
ELISA Kit Range0.55-400pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)78-10286
EDTA Plasma (n=5)94-10197
Heparin Plasma (n=5)98-105101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 6 (IL6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 6 (IL6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Il6 Microplate96 Wells (12 x 8 Well strips)
Il6 Lyophilized Standard2
100X Il6 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity0.55 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIl6
Gene Full Nameinterleukin 6
Alias SymbolsB-cell hybridoma growth factor;Il;Il-6;interleukin HP-1;interleukin-6.
NCBI Gene Id16193
Protein NameInterleukin-6
Description of TargetCytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation.
Uniprot IDP08505
Protein Accession #NP_001300983
Nucleotide Accession #NM_001314054

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