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> Imipramine N-oxide [6829-98-7]

Imipramine N-oxide [6829-98-7]

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Imipramine N-oxide is the metabolite of Imipramine. Imipramine is a tertiary amine tricyclic antidepressant.

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Imipramine N-oxide Estructura química

Imipramine N-oxide Estructura química

No. CAS : 6829-98-7

This product is a controlled substance and not for sale in your territory.

Based on 1 publication(s) in Google Scholar

Descripciòn

Imipramine N-oxide is the metabolite of Imipramine. Imipramine is a tertiary amine tricyclic antidepressant[1][2].

Peso molecular

296.41

Fòrmula

C19H24N2O
No. CAS

6829-98-7
Appearance

Solid

Color

White to off-white

SMILES

O=N(C)(C)CCCN1C2=CC=CC=C2CCC3=CC=CC=C13
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvente y solubilidad
In Vitro: 

DMSO : 100 mg/mL (337.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3737 mL 16.8685 mL 33.7371 mL
5 mM 0.6747 mL 3.3737 mL 6.7474 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Calculadora de molaridad

  • Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.43 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (8.43 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación

Purity: 99.87%

COA (257 KB) HNMR (206 KB) LCMS (264 KB)

Instrucciones de manejo (2659 KB)
Referencias

  • [1]. Bickel MH, et al. Metabolic interconversions between imipramine, its N-oxide, and its desmethyl derivative in rat tissues in vitro. Biochem Biophys Res Commun. 1968;33(6):1012-1018.  [Content Brief]

    [2]. Fayez R, et al. Imipramine. [Updated 2021 Nov 20]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2022 Jan-.

  • [1]. Bickel MH, et al. Metabolic interconversions between imipramine, its N-oxide, and its desmethyl derivative in rat tissues in vitro. Biochem Biophys Res Commun. 1968;33(6):1012-1018.

    [2]. Fayez R, et al. Imipramine. [Updated 2021 Nov 20]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2022 Jan-.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3737 mL 16.8685 mL 33.7371 mL 84.3426 mL
5 mM 0.6747 mL 3.3737 mL 6.7474 mL 16.8685 mL
10 mM 0.3374 mL 1.6869 mL 3.3737 mL 8.4343 mL
15 mM 0.2249 mL 1.1246 mL 2.2491 mL 5.6228 mL
20 mM 0.1687 mL 0.8434 mL 1.6869 mL 4.2171 mL
25 mM 0.1349 mL 0.6747 mL 1.3495 mL 3.3737 mL
30 mM 0.1125 mL 0.5623 mL 1.1246 mL 2.8114 mL
40 mM 0.0843 mL 0.4217 mL 0.8434 mL 2.1086 mL
50 mM 0.0675 mL 0.3374 mL 0.6747 mL 1.6869 mL
60 mM 0.0562 mL 0.2811 mL 0.5623 mL 1.4057 mL
80 mM 0.0422 mL 0.2109 mL 0.4217 mL 1.0543 mL
100 mM 0.0337 mL 0.1687 mL 0.3374 mL 0.8434 mL
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Imipramine N-oxide Related Classifications

  • Metabolic Enzyme/Protease
  • Drug Metabolite
Help & FAQs

Keywords:

Imipramine N-oxide6829-98-7Drug MetaboliteImipraminemetabolitetertiary amine tricyclicantidepressantInhibitorinhibitorinhibit