Disease:

Glioblastoma

Origin:

The LN-229 cell line was established in 1979 from cells taken from a patient with right frontal parieto-occipital glioblastoma.

Species:

Homo sapiens, human

Tissue:

Brain/right frontal parieto-occipital cortex

Morphology:

Epithelial

Properties:

Adherent

Patient:

Caucasian, female, 60 years of age

Medium:

AddexBio-formulated DMEM (C0003-01) + 5% FBS

Subculture:

Subculture at a ratio of 1:4 to 1:6 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C.

Freezing Medium:

Complete growth culture medium supplemented with 5% (v/v) DMSO