Images
Flow Cytometry
Application

Flow Cytometry

Flow cytometric analysis of HEK293T cells unstained K252a treated cells as negative control (blue) or stained and treated with K252a (red) or treated with UV+TPA (green) using MAP2K3/MAP2K6 (phospho S189/S207) monoclonal antibody (PE).

  • Specifications

    Product Description

    Rabbit monoclonal antibody raised against synthetic phosphopeptide of human MAP2K3/MAP2K6.

    Immunogen

    A synthetic phosphopeptide corresponding to residues surrounding S189/S207 of human MAP2K3/MAP2K6.

    Host

    Rabbit

    Reactivity

    Human

    Form

    Liquid

    Conjugation

    PE

    Purification

    Protein A/G Purification

    Isotype

    IgG1k

    Recommend Usage

    Flow Cytometry (5 uL/106 cells or 0.05 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, pH 7.4 (0.2% BSA, 0.09% sodium azide).

    Storage Instruction

    Store at 2-8°C.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Flow Cytometry

    Flow cytometric analysis of HEK293T cells unstained K252a treated cells as negative control (blue) or stained and treated with K252a (red) or treated with UV+TPA (green) using MAP2K3/MAP2K6 (phospho S189/S207) monoclonal antibody (PE).
  • Gene Info — MAP2K3

    Entrez GeneID

    5606

    Gene Name

    MAP2K3

    Gene Alias

    MAPKK3, MEK3, MKK3, PRKMK3

    Gene Description

    mitogen-activated protein kinase kinase 3

    Omim ID

    602315

    Gene Ontology

    Hyperlink

    Gene Summary

    The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq

    Other Designations

    MAP kinase kinase 3|MAPK/ERK kinase 3|OTTHUMP00000166044|dual specificity mitogen activated protein kinase kinase 3

  • Gene Info — MAP2K6

    Entrez GeneID

    5608

    Gene Name

    MAP2K6

    Gene Alias

    MAPKK6, MEK6, MKK6, PRKMK6, SAPKK3

    Gene Description

    mitogen-activated protein kinase kinase 6

    Omim ID

    601254

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. [provided by RefSeq

    Other Designations

    protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6)

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