MAPK2 Blocking Peptide peptide

Formulation:	Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice.
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	MAPKAPK2
Gene ID:	9261
Uniprot ID:	MAPK2_HUMAN
Immunogen Region:	300-350
Immunogen:	Synthetic peptide taken within amino acid region 300-350 on human Mitogen-activated protein kinase 2.

Tissue Specificity	Expressed in all tissues examined.
Post Translational Modifications	Sumoylation inhibits the protein kinase activity. Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-222, Ser-272 and Thr-334.
Function	Stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, CEP131, ELAVL1, HNRNPA0, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Phosphorylates HSF1.leading to the interaction with HSP90 proteins and inhibiting HSF1 homotrimerization, DNA-binding and transactivation activities. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to the dissociation of HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impairment of their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to the regulation of the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity, leading to inhibition of dependent degradation of ARE-containing transcripts. Phosphorylates CEP131 in response to cellular stress induced by ultraviolet irradiation which promotes binding of CEP131 to 14-3-3 proteins and inhibits formation of novel centriolar satellites. Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilization of GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3.
Peptide Name	Map Kinase-Activated Protein Kinase 2 Mapk-Activated Protein Kinase 2 Mapkap Kinase 2 Mapkap-K2 Mapkapk-2 Mk-2 Mk2
Database Links	Reactome: R-HSA-171007 Reactome: R-HSA-199920 Reactome: R-HSA-2142691 Reactome: R-HSA-2559580 Reactome: R-HSA-3371453 Reactome: R-HSA-4420097 Reactome: R-HSA-450302 Reactome: R-HSA-450385 Reactome: R-HSA-450513 Reactome: R-HSA-5357905
Cellular Localisation	Cytoplasm Nucleus Phosphorylation And Subsequent Activation Releases The Autoinhibitory Helix Resulting In The Export From The Nucleus Into The Cytoplasm
Alternative Peptide Names	Map Kinase-Activated Protein Kinase 2 protein Mapk-Activated Protein Kinase 2 protein Mapkap Kinase 2 protein Mapkap-K2 protein Mapkapk-2 protein Mk-2 protein Mk2 protein MAPKAPK2 protein

Information sourced from Uniprot.org

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