Matrix Metalloproteinase 9 (Matrix Metallopeptidase 9, MMP-9, MMP9, 92kD Gelatinase, 92kD Type IV Collagenase, CLG4B, Gelatinase B, GELB, MANDP2)

The MMP’s are zinc-dependent endopeptidases responsible for degrading the extracellular matrix. They are also involved in cell proliferation, migration, differentiation and apoptosis. Most MMP’s are synthesized as inactive zymogens and a propeptide region must be cleaved off before the enzyme becomes active. Their expression is increased dramatically in a variety of cancer types, where it indicates invasive disease and a poor prognosis. MMP-9 is a gelatinase, cleaving type IV collagen and gelatin. The gelatinases have an additional gelatin-binding domain inserted in the catalytic domain. MMP-9, alongside other MMPs, plays a role in normal tissue remodeling such as embryonic development, ovulation, mammary gland involution and wound healing. It is important in the early stages of tumor invasion as it degrades the type IV collagen in the basement membrane.||Applications:|Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.||Recommended Dilution:|Immunohistochemistry (paraffin): 1:25-1:100|Optimal dilutions to be determined by the researcher.||Immunohistochemistry Positive Control:|Esophageal adenocarcinoma||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.