Disease:

Adenocarcinoma

Origin:

Established in 1977 from the pleural effusion of a 51-year-old black woman with metastatic adenocarcinoma of the breast; these cells carry a p53 point mutation at codon 273 leading to Arg-His substitution

Species:

Homo sapiens

Tissue:

Breast, mammary glands

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

modal number = 64; range = 60 to 67.

Patient:

Female, Black, 51 yrs of age

Medium:

AddexBio-formulated Leibovitz's L-15 Medium (C0006-01) + 10% FBS

Subculture:

Seed cells at 1-2 x 10^6 cells in T75 flask; split confluent culture 1:3 to 1:10 every 3-5 days using trypsin/EDTA; without CO2 (or use non-vented flask and keep the lid closed); 37°C

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO