MG-63
Referencia C0004007
embalaje : OneFrozenvial
Disease:
Osteosarcoma
Origin:
From an osteosarcoma of a 14 year old male. Produces high yields of interferon following superinduction with polyinosinic acid-polycytidilic acid, cyclohexamide and actinomycin. Antigenically, MG-63 interferon is more closely related to human fibroblast than to leukocyte interferon.
Species:
Homo sapiens, human Bone Fibroblast Adherent This is a hypotriploid human cell line. The modal chromosome number was 66 occurring in 44% of cells. The rate of cells with higher ploidies was 2.0%. Eighteen to 19 marker chromosomes were common to all cells. Caucasian, Male, 14 years of age AddexBio-formulated EMEM (C0005-01) + 10% FBS Subculture at a ratio of 1:4 to 1:8 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C. Complete growth culture medium supplemented with 5% (v/v) DMSO I Bacteria: Negative Yeast: Negative Mycoplasma: Negative HIV: Negative Hepatitis B: Negative Amelogenin: X,Y CSF1PO: 10,12 D13S317: 11 D16S539: 11 D5S818: 11,12 D7S820: 10 THO1:9.3 TPOX: 8,11 vWA: 16,19 If use of this culture results in a scientific publication, it should be cited in the publication as: MG-63 (AddexBio C0004007) Tissue:
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References for MG-63 cells:
1. Billiau A, Edy VG, Heremans H, Van Damme J, Desmyter J, Georgiades JA, De Somer P. Human interferon: mass production in a newly established cell line, MG-63. Antimicrob Agents Chemother. 1977;12(1):11-15. PMID: 883813