MHC Class II Monomorphic (PE)

Referencia 227449-100T

embalaje : 100Tests

Marca : US Biological

Solicitar más información

Contact local distributor :


Teléfono : +1 850 650 7790


227449 MHC Class II Monomorphic (PE)

Clone Type
Polyclonal
Host
mouse
Source
equine
Isotype
IgG
Grade
Affinity Purified
Applications
FC
Crossreactivity
Eq
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Recognizes monomorphic equine MHC Class II and was classified at the International Equine Leucocyte Antigen Workshop. Reacts with all equine B cells and 95% of equine T cells.||The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In horses, this is referred to as the equine leukocyte antigen (ELA) region.||Applications: |Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilution:|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG|Clone No: CVS20|Host: mouse|Source: equine|Concentration: As reported |Form: Supplied as a lyophilized powder in PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Reconstitute with 1ml ddH2O. Labeled with R-Phycoerythrin (PE). |Purity: Purified by Protein A affinity chromatography.|Immunogen: 3132 cells.|Specificity: Recognizes equine MHC Class II Monomorphic.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
3132 cells.
Form
Supplied as a lyophilized powder in PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Reconstitute with 1ml ddH2O. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes equine MHC Class II Monomorphic.
References
1. Kydd, J.H. & Antczak. D.F. (1991) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991 Equine Immunol. 4: 5. 2. Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995. Vet. Immunol. Immunopathol. 62: 101-143. 3. Weiss, D. et al. (2001) Regulation of expression of major histocompatibility antigens by bovine macrophages infected with Mycobacterium avium subsp. paratuberculosis or Mycobacterium avium subsp. avium. Infect. Immun. 69(2): 1002-1008. 4. Out, T. et al. (2002) Local T-cell activation after segmental allergen challenge in the lungs of allergic dogs. Immunology. 105: 499-508. 5. Carrade, D.D. et al. (2011) Clinicopathologic findings following intra-articular injection of autologous and allogeneic placentally derived equine mesenchymal stem cells in horses. Cytotherapy. 13: 419-30. 6. Catchpole, B. et al. (2002) Generation of blood-derived dendritic cells in dogs with oral malignant melanoma. J Comp Pathol. 126: 238-41. 7. Weiss, D.J. et al. (2006) Mucosal immune response in cattle with subclinical Johne's disease. Vet Pathol. 43: 127-35. 8. Weiss, D.J. (2001) Evaluation of proliferative disorders in canine bone marrow by use of flow cytometric scatter plots and monoclonal antibodies. Vet Pathol. 38: 512-8. 9. Sassa, Y. et al. (2010) Bovine macrophage degradation of scrapie and BSE PrPSc Vet Immunol Immunopathol. 133: 33-9. 10. Carrade, D.D. et al. (2012) Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells. Cell Medicine. 4: 1-11.