Reactivos e instrumentos para inmunología, biología celular y biología molecular.
 
> Milenia® GenLine HybriDetect

Milenia® GenLine HybriDetect

Imprimir Imprimir
Por favor conéctese

Referencia MGHD1-5

embalaje : 5x100tests

Marca : Milenia Biotec

Contact local distributor :


Teléfono : +1 850 650 7790

( excludes VAT and shipping costs)
100

The HybriDetect is a simple and quick tool to develop your own rapid test. Various molecules can be detected: Proteins, Antibodies, Genetic amplicons

Please note, that you may have to pay country-specific taxes and duties.

Safety Data Sheet
Overview
Name of Product
HybriDetect - Universal Lateral Flow Assay Kit
Catalog Number
MGHD 1
Short Info

The HybriDetect is a simple and quick tool to develop your own rapid test. Various molecules can be detected: Proteins, Antibodies, Genetic amplicons

Please note, that you may have to pay country-specific taxes and duties.

Method/Platform
Lateral flow, sandwich or competetive
Range/Assay Sensivity
5 pg DNA, eqivalent to agarose gel electrophoresis
Test Principle

HybriDetect is a ready-to-use, universal test strip (dipstick) based on lateral flow technology using gold particles. The dipstick can be used to develop qualitative or quantitative rapid test systems for a wide range of analytes such as antibodies, gene amplification products or proteins . The results can be interpreted qualitative or quantitative.

 

 

Documents
Protocol
Brief Instructions

Click here for the Instructions for Use

Storage

2-8°C

Components

HybriDetect dipsticks, HybriDetect assay buffer

References
  • A chemical-enhanced system for CRISPR-Based nucleic acid detection (2021)
    Li, Z., Zhao, W., Ma, S., Li, Z., Yao, Y., & Fei, T.
  • A CRISPR-based assay for the detection of opportunistic infections post-transplantation and for the monitoring of transplant rejection. (2020)
    Kaminski M., Alcantar M., Lape I., et.al.
  • A CRISPR-based lateral flow assay for plant genotyping and pathogen diagnostics (2022)
    Sánchez, E., Ali, Z., Islam, T., Mahfouz, M., & Mahfouz, M. M.
  • A CRISPR-Cas12a-based diagnostic method for multiple genotypes of severe fever with thrombocytopenia syndrome virus (2022)
    Ju, B., P., Rae, J., Y., Heo, S. T., Kim, M., Lee, K. H., & Songid, Y.-J.
  • A CRISPR-Cas12a-based platform facilitates the detection and serotyping of Streptococcus suis serotype 2 (2024)
    Wang, L., Sun, J., Zhao, J., Bai, J., Zhang, Y., Zhu, Y., Zhang, W., Wang, C., Langford, P. R., Liu, S., & Li, G.
  • A Fast and Simple DNA Mini-barcoding and RPA Assay Coupled with Lateral Flow Assay for Fresh and Canned Mackerel Authentication (2022)
    Frigerio, J., Gorini, T., Palumbo, C., de Mattia, F., Labra, M., & Mezzasalma, V.
  • A field-deployable method for single and multiplex detection of DNA or RNA from pathogens using Cas12 and Cas13 (2021)
    Li, L., Duan, C., Weng, J., Qi, X., Liu, C., Li, X., Zhu, J., & Xie, C.
  • A Lateral Flow Assay for Nucleic Acid Detection Based on Rolling Circle Amplification Using Capture Ligand-Modified Oligonucleotides (2022)
    Lee, H. N., Lee, J., Yoo, ·, Kang, K., Joo, ·, Lee, H., Yang, S., & Chung, H. J.
  • A lateral flow dipstick combined with reverse transcription recombinase polymerase amplification for rapid and visual detection of the bovine respirovirus (2018)
    Zhao, G., Wang, H., Hou, P., Xia, X., & He, H.
  • A lateral flow dipstick combined with reverse transcription recombinase polymerase amplification for rapid and visual detection of the BVDV and BPIV3 (2022)
    Yang, S., Wang, Q. Y., Tan, B., Shi, P. F., Qiao, L. J., Li, Z. J., Liu, K. X., Cao, Z. G., Zhang, S. Q., & Sun, F. Y.
  • A microfluidic-integrated lateral flow recombinase polymerase amplification (MI-IF-RPA) assay for rapid COVID-19 detection (2021)
    Liu, D., Shen, H., Zhang, Y., Shen, D., Zhu, M., Song, Y., Zhu, Z., & Yang, C.
  • A Multiplexed Cas13-Based Assay with Point-of-Care Attributes for Simultaneous COVID-19 Diagnosis and Variant Surveillance. (2022)
    Patchsung, M., Homchan, A., Aphicho, K., Suraritdechachai, S., Wanitchanon, T., Pattama, A., Sappakhaw, K., Meesawat, P., Wongsatit, T., Athipanyasilp, A., Jantarug, K., Athipanyasilp, N., Buahom, J., Visanpattanasin, S., Niljianskul, N., Chaiyen, P., Tinikul, R., Wichukchinda, N., Mahasirimongkol, S., Uttamapinant, C.
  • A new method for the rapid detection of Atlantic cod (Gadus morhua), Pacific cod (Gadus macrocephalus), Alaska pollock (Gadus chalcogrammus) and ling (Molva molva) using a lateral flow dipstick assay. Food Chem. 2017; 233, 182-189 (2017)
    Taboada L. et al.
  • A novel rapid detection of Senecavirus A using recombinase polymerase amplification (RPA) coupled with lateral flow (LF) dipstrip (2022)
    Wang, H., Dong, J., Zhang, T., Wang, F., Yang, R., Zhang, Y., & Zhao, X.
  • A Novel Test to Diagnose Canine Visceral Leishmaniasis at the Point of Care. Am. J. Med Hyg. 2015, 93 (5), 970-975 (2015)
    Castellanos-Gonzalez A et al.
  • A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs (2023)
    Yan, H., Wen, Y., Tian, Z., Hart, N., Han, S., Hughes, S. J., & Zeng, Y.
  • A one-tube rapid visual CRISPR assay for the field detection of Japanese encephalitis virus (2022)
    Xu, B., Gong, P., Zhang, Y., Wang, Y., Tao, D., Fu, L., Khazalwa, E. M., Liu, H., Zhao, S., Zhang, X., & Xie, S.
  • A Paper and Plasitc Device for the Combined Isothermal Amplification and Lateral Flow Detection of Plasmodium DNA. Malar. J. 2015; 14, 472 (2015)
    Cordray MS et al.
  • A portable CRISPR Cas12a based lateral flow platform for sensitive detection of Staphylococcus aureus with double insurance (2021)
    Qian J., Huang D., Ni D., Zhao J., Shi Z., Fang M. & Xu Z.
  • A protocol for detection of COVID-19 using CRISPR diagnostics. (2020)
    Zhang F., Abudayyeh O. O., Gootenberg J.S., Mathers L.
  • A protocol for rapid detection of the 2019 novel coronavirus SARS-CoV-2 using CRISPR diagnostics : SARS-CoV-2 DETECTR. (2020)
    Broughton J. P., Wayne D., Fasching C. L., Singh J., Charles Y., Chen J. S.
  • A rapid and high-throughput Helicobacter pylori RPA-CRISPR/Cas12a-based nucleic acid detection system (2022)
    Liu, H., Wang, J., Hu, X., Tang, X., & Zhang, C.
  • A rapid and precise diagnostic method for detecting the pinewood nematode Bursaphelenchus xylophilus by loop-mediated isothermal amplification. Phytopathology, 99(12), 1365–1369. (2009)
    Kikuchi, T., Aikawa, T., Oeda, Y., Karim, N., & Kanzaki, N.
  • A rapid and visual detection assay for Clonorchis sinensis based on recombinase polymerase amplification and lateral flow dipstick (2023)
    Ma, X., Bai, X., Li, H., Ding, J., Zhang, H., Qiu, Y., Wang, J., Liu, X., Liu, M., Tang, B., & Xu, N.
  • A rapid detection of tomato yellow leaf curl virus using recombinase polymerase amplification-lateral flow dipstick assay (2021)
    Zhou, Y., Zheng, H. Y., Jiang, D. M., Liu, M., Zhang, W., & Yan, J. Y.
  • A rapid, equipment-free method for detecting Phytophthora infestans in the field using a lateral flow strip-based recombinase polymerase amplification assay. (2020)
    Xinyu L., Zheng Y., Zhang F.
  • A recombinase polymerase amplification lateral flow dipstick assay for rapid detection of the quarantine citrus pathogen in China, Phytophthora hibernalis (2019)
    Dai T, Hu T, Yang X, Shen D, Jiao B, Tian W, Xu Y
  • A recombinase polymerase amplification lateral flow dipstick for field diagnosis of bovine leukemia virus infection and its effectiveness compared to iiPCR and ELISA (2018)
    Tu PA, Shiu JS, Lai FY, Chen YH, Shiau JW, Pang VF, Wang PH
  • A Saliva-Based RNA Extraction-Free Workflow Integrated With Cas13a for SARS-CoV-2 Detection (2021)
    Azmi, I., Faizan, M. I., Kumar, R., Raj Yadav, S., Chaudhary, N., Kumar Singh, D., Butola, R., Ganotra, A., Datt Joshi, G., Deep Jhingan, G., Iqbal, J., Joshi, M. C., & Ahmad, T.
  • A sample-to-answer COVID-19 diagnostic device based on immiscible filtration and CRISPR-Cas12a-assisted detection (2022)
    Ngamsom, B., Iles, A., Kamita, M., Kimani, R., Wakaba, P., Rodriguez-Mateos, P., Mungai, M., Dyer, C. E., Walter, C., Gitaka, J., & Pamme, N.
  • A Scalable, Easy-to-Deploy Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material (2021)
    Rauch, J. N., Valois, E., Solley, S. C., Braig, F., Lach, R. S., Audouard, M., Ponce-Rojas, J. C., Costello, M. S., Baxter, N. J., Kosik, K. S., Arias, C., Acosta-Alvear, D., & Wilson, M. Z.
  • Advanced Multiplex Loop Mediated Isothermal Amplification (mLAMP) Combined with Lateral Flow Detection (LFD) for Rapid Detection of Two Prevalent Malaria Species in India and Melting Curve Analysis (2022)
    Sharma S, Kumar S, Ahmed MZ, Bhardwaj N, Singh J, Kumari S, Savargaonkar D, Anvikar AR, Das J
  • Allosteric control of type I-A CRISPR-Cas3 complexes and establishment as effective nucleic acid detection and human genome editing tools (2022)
    Hu, C., Ni, D., Nam, K. H., Majumdar, S., McLean, J., Stahlberg, H., Terns, M. P., & Ke, A.
  • An enhanced isothermal amplification assay for viral detection. (2020)
    Qian J., Boswell S., Chidley C., et. al.
  • An enhanced isothermal amplification assay for viral detection. (2020)
    Qian J. et al
  • An enhanced method for nucleic acid detection with CRISPR-Cas12a using phosphorothioate modified primers and optimized gold-nanopaticle strip (2021)
    Gong, J., Kan, L., Zhang, X., He, Y., Pan, J., Zhao, L., Li, Q., Liu, M., Tian, J., Lin, S., Lu, Z., Xue, L., Wang, C., & Tang, G.
  • An Inexpensive CRISPR-Based Point-of-Care Test for the Identification of Meat Species and Meat Products (2022)
    Tao, D., Xiao, X., Lan, X., Xu, B., Wang, Y., Khazalwa, E. M., Pan, W., Ruan, J., Jiang, Y., Liu, X., Li, C., Ye, R., Li, X., Xu, J., Zhao, S., & Xie, S.
  • An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannis ssp. Infections. PLoS Negl. Trop. Dis. 2016; 10 (4) (2016)
    Saldarriaga O. A. et al.
  • An Integrated Paper Microfluidic Device Based on Isothermal Amplification for Simple Sample-to-Answer Detection of Campylobacter jejuni (2023)
    Chen, Y., Hu, Y., & Lu, X.
  • An RT-RPA-Cas12a platform for rapid and sensitive detection of tilapia lake virus (2022)
    Sukonta, T., Senapin, S., Taengphu, S., Hannanta-anan, P., Kitthamarat, M., Aiamsa-at, P., & Chaijarasphong, T.
  • An RxLR Effector Gene and New Biomarker in A Recombinase Polymerase Amplification Assay for Rapid Detection of Phytophthora cinnamomi. (2020)
    Dai T., Wang A., Yang X., Yu X., Tain W., Xu Y
  • An ultrasensitive and point-of-care sensor for the telomerase activity detection (2021)
    Chen, X., Deng, Y., Cao, G., Liu, X., Gu, T., Feng, R., Huo, D., Xu, F., & Hou, C.
  • Application of Hybridization Chain Reaction/CRISPR-Cas12a for the Detection of SARS-CoV-2 Infection (2023)
    Sagoe, K. O., Kyama, M. C., Maina, N., Kamita, M., Njokah, M., Thiong’o, K., Kanoi, B. N., Wandera, E. A., Ndegwa, D., Kinyua, D. M., & Gitaka, J.
  • Application of loop-mediated isothermal amplification combined with colorimetric and lateral flow dipstick visualization as the potential point-of-care testing for Corynebacterium diphtheria. (2020)
    Zasada A., Wiatrzyk A., Czajka U., et. al.
  • Application of Rapid and Reliable Detection of Cymbidium Mosaic Virus by Reverse Transcription Recombinase Polymerase Amplification Combined with Lateral Flow Immunoassay (2022)
    Kim, D. H., Jeong, R. D., Choi, S., Ju, H. J., & Yoon, J. Y.
  • Application of the PCR and allele-specific PCR-nucleic acid lateral flow immunoassay for the detection of the SRY gene and the G1138A mutation in the FGFR3 gene for the human DNA (2023)
    Sukjai, A., Puangmali, T., Wichajarn, K., & Monthatong, M.
  • Aptamers for SARS-CoV-2: Isolation, Characterization, and Diagnostic and Therapeutic Developments (2022)
    Amini, R., Zhang, Z., Li, J., Gu, J., Brennan, J. D., & Li, Y.
  • Automated, portable, and high-throughput fluorescence analyzer (APHF-analyzer) and lateral flow strip based on CRISPR/Cas13a for sensitive and visual detection of SARS-CoV-2 (2022)
    Cao, G., Huo, D., Chen, X., Wang, X., Zhou, S., Zhao, S., Luo, X., & Hou, C.
  • Backpack PCR: A point-of-collection diagnostic platform for the rapid detection of Brugia parasites in mosquitoes (2018)
    Zaky WI, Tomaino FR, Pilotte N, Laney SJ, Williams SA
  • Bio-SCAN V2: A CRISPR/dCas9-based lateral flow assay for rapid detection of theophylline (2023)
    Jiang, W., Aman, R., Ali, Z., & Mahfouz, M.
  • Bio-SCAN: A CRISPR/dCas9-Based Lateral Flow Assay for Rapid, Specific, and Sensitive Detection of SARS-CoV-2 (2021)
    Ali, Z., Sánchez, E., Tehseen, M., Mahas, A., Marsic, T., Aman, R., Sivakrishna Rao, G., Alhamlan, F. S., Alsanea, M. S., Al-Qahtani, A. A., Hamdan, S., & Mahfouz, M.
  • Bio-SCAN: A CRISPR/dCas9-Based Lateral Flow Assay for Rapid, Specific, and Sensitive Detection of SARS-CoV-2 (2022)
    Ali, Z., Sánchez, E., Tehseen, M., Mahas, A., Marsic, T., Aman, R., Sivakrishna Rao, G., Alhamlan, F. S., Alsanea, M. S., Al-Qahtani, A. A., Hamdan, S., & Mahfouz, M.
  • Bushmeat Species Identification: Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow (LF) Strip for Identification of Formosan Reeves' Muntjac (Muntiacus reevesi micrurus) (2021)
    Hsu, Y. H., Yang, W. C., & Chan, K. W.
  • Cas protein diagnostics for pathogen nucleic acids (2023)
    Das, A.
  • Cas12a and Lateral Flow Strip-Based Test for Rapid and Ultrasensitive Detection of Spinal Muscular Atrophy (2021)
    Zhang, C., Li, Z., Chen, M., Hu, Z., Wu, L., Zhou, M., & Liang, D.
  • Cas12a-Based Diagnostics for Potato Purple Top Disease Complex Associated with Infection by ‘Candidatus Phytoplasma trifolii’-Related Strains (2022)
    Wheatley, M. S., Wang, Q., Wei, W., Bottner-Parker, K. D., Zhao, Y., & Yang, Y.
  • Cas12a-based on-site and rapid nucleic acid detection of african swine fever (2019)
    Bai J, Lin H, Li H, Zhou Y, Liu J, Zhong G, Wu L, Jiang W, Du H, Yang J, Xie Q, Huang L
  • Cas12a/Guide RNA-Based Platforms for Rapidly and Accurately Identifying Staphylococcus aureus and Methicillin-Resistant S. aureus (2023)
    Cao, X., Chang, Y., Tao, C., Chen, S., Lin, Q., Ling, C., Huang, S., Zhang, H., & Rogovskyy, A. S.
  • CLEVER assay: A visual and rapid RNA extraction-free detection of SARS-CoV-2 based on CRISPR-Cas integrated RT-LAMP technology (2022)
    Bhatt, A., Fatima, Z., Ruwali, M., Misra, C. S., Rangu, S. S., Rath, D., Rattan, A., & Hameed, S.
  • Clinical validation of engineered CRISPR/Cas12a for rapid SARS-CoV-2 detection (2022)
    Nguyen, L. T., Rananaware, S. R., Pizzano, B. L. M., Stone, B. T., & Jain, P. K.
  • Combining CRISPR-Cas12a with Terminal Deoxynucleotidyl Transferase dependent reporter elongation for pathogen detection using Lateral Flow Test Strips (2022)
    Berghuis, N. F., Mars-Groenendijk, R., Busker, R. W., Paauw, A., & van Leeuwen, H. C.
  • Combining recombinase polymerase amplification and DNA-templated reaction for SARS-CoV-2 sensing with dual fluorescence and lateral flow assay output (2022)
    Farrera-Soler, L., Gonse, A., Kim, K. T., Barluenga, S., & Winssinger, N.
  • Comparative diagnostic performance of a Cas13-based assay for de-tecting COVID-19 cases in Al-Dewaniyah province, Iraq (2023)
    Alkaebi, F., Tahmasebi, P., & Alsultan, A.
  • Comparative evaluation of a novel recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) assay, LAMP, conventional PCR, and leaf-disc baiting methods for detection of Phytophthora sojae (2019)
    Dai T, Yang X, Hu T, Jiao B, Xu Y, Zheng X, Shen D
  • COVID-19 infection diagnosis: Potential impact of isothermal amplification technology to reduce community transmission of SARS-CoV-2. (2020)
    James A., Alwneh J.,
  • CRISPR-based diagnostics detects invasive insect pests (2023)
    Shashank, P. R., Parker, B. M., Rananaware, S. R., Plotkin, D., Couch, C., Yang, L. G., Nguyen, L. T., Prasannakumar, N. R., Braswell, W. E., Jain, P. K., & Kawahara, A. Y.
  • CRISPR-based platform for carbapenemases and emerging viruses detection using Cas12a (Cpf1) effector nuclease. (2020)
    Curti L., Pereyra-Bonnet F., Repizo G., et. al.
  • CRISPR-based platform for rapid, sensitive and field-deployable detection of scale drop disease virus in Asian sea bass (Lates calcarifer) (2022)
    Sukonta, T., Senapin, S., Meemetta, W., & Chaijarasphong, T.
  • CRISPR-based surveillance for COVID-19 using genomically-comprehensive machine learning design. (2020)
    Metsky H. C., Freije C. A., Kosoko-Thoroddsen T-S. F., Sabeti P.C., Myhrvold C.,
  • CRISPR-based, genotype-specific detection of yellow head virus genotype 1 with fluorescent, lateral flow and DNAzyme-assisted colorimetric readouts (2023)
    Aiamsa-at, P., Nonthakaew, N., Phiwsaiya, K., Senapin, S., & Chaijarasphong, T.
  • CRISPR-Cas fluorescent cleavage assay coupled with recombinase polymerase amplification for sensitive and specific detection of Enterocytozoon hepatopenaei. (2020)
    Kanitchinda S., Srisala J., Suebsing R., et. al.
  • CRISPR-Cas-amplified urine biomarkers for multiplexed and portable cancer diagnostics (2020)
    Hao L., Zhao R., Ngambenjawong C., et. al.
  • CRISPR-Cas12-based detection of SARS-CoV-2, Nature Biotechnology (2020)
    Broughton J.P., Deng X., Yu G., Fasching C.L., Servellita V., Singh J., Miao X., Streithorst J.A., Granados A., Sotomayor-Gonzales A., Zorn K., Gopez A., Hsu E., Gu W., Miller S., Pan C.Q., Guevara H., Wadford D.A., Chen J.S., Chiu C.Y.
  • CRISPR-Cas12-based detection of SARS-CoV-2. (2020)
    Broghton J.P., et al.
  • CRISPR-Cas12a-Based Diagnostic Method for Japanese Encephalitis Virus Genotypes I, III, and V (2023)
    Kwak, N., Park, B. J., & Song, Y.-J.
  • CRISPR-Cas13a-Based Assay for Accurate Detection of OXA-48 and GES Carbapenemases (2023)
    Ortiz-Cartagena, C., Pablo-Marcos, D., Fernández-García, L., Blasco, L., Pacios, O., Bleriot, I., Siller, M., López, M., Fernández, J., Aracil, B., Fraile-Ribot, P. A., García-Fernández, S., Fernández-Cuenca, F., Hernández-García, M., Cantón, R., Calvo-Montes, J., & Tomás, M.
  • CRISPR-Cas13a-based detection method for avian influenza virus (2023)
    Wu, Y., Zhan, J., Shan, Z., Li, Y., Liu, Y., Li, Y., Wang, Y., Liu, Z., Wen, X., & Wang, X.
  • CRISPR-Cas3-based diagnostics for SARS-CoV-2 and influenza virus (2022)
    Yoshimi, K., Takeshita, K., Yamayoshi, S., Shibumura, S., Yamauchi, Y., Yamamoto, M., Yotsuyanagi, H., Kawaoka, Y., & Mashimo, T.
  • CRISPR-ENHANCE: An enhanced nucleic acid detection platform using Cas12a (2021)
    Nguyen, L. T., Gurijala, J., Rananaware, S. R., Pizzano, B., Stone, B. T., & Jain, P. K.
  • CRISPR/Cas12-Based Ultra-Sensitive and Specific Point-of-Care Detection of HBV (2021)
    Ding, R., Long, J., Yuan, M., Zheng, X., Shen, Y., Jin, Y., Yang, H., Li, H., Chen, S., & Duan, G.
  • CRISPR/Cas12a-Based Detection Platform for Early and Rapid Diagnosis of Scrub Typhus (2023)
    Bhardwaj, P., Nanaware, N. S., Behera, S. P., Kulkarni, S., Deval, H., Kumar, R., Dwivedi, G. R., Kant, R., & Singh, R.
  • CRISPR/Cas12a-Based Ultrasensitive and Rapid Detection of JAK2 V617F Somatic Mutation in Myeloproliferative Neoplasms (2021)
    Chen, M., Zhang, C., Hu, Z., Li, Z., Li, M., Wu, L., Zhou, M., & Liang, D.
  • CRISPR/Cas9-Based Lateral Flow and Fluorescence Diagnostics (2021)
    Osborn, M. J., Bhardwaj, A., Bingea, S. P., Knipping, F., Feser, C. J., Lees, C. J., Collins, D. P., Steer, C. J., Blazar, B. R., & Tolar, J.
  • CRISPRs in the human genome are differentially expressed between malignant and normal adjacent to tumor tissue (2022)
    van Riet, J., Saha, C., Strepis, N., Brouwer, R. W. W., Martens-Uzunova, E. S., van de Geer, W. S., Swagemakers, S. M. A., Stubbs, A., Halimi, Y., Voogd, S., Tanmoy, A. M., Komor, M. A., Hoogstrate, Y., Janssen, B., Fijneman, R. J. A., Niknafs, Y. S., Chinnaiyan, A. M., van IJcken, W. F. J., van der Spek, P. J. Louwen, R.
  • Detection assay for HPV16 and HPV18 by loop‑mediated isothermal amplification with lateral flow dipstick (2017)
    Kumvongpin R, Jearanaikoon P, Wilailuckana C, Sae‑Ung N, Prasongdee P, Daduang S, Wongsena M, Boonsiri P, Kiatpathomchai W, Swangvaree SS, Sandee A, Daduang J
  • Detection of a single nucleotide polymorphism for insecticide resistance using recombinase polymerase amplification and lateral flow dipstick detection (2022)
    Ahmed, M., Pollak, N. M., Devine, G. J., & Macdonald, J.
  • Detection of Cryptosporidium Species in Kenya Using Lateral Flow Loop-Mediated Isothermal Amplification (2020)
    Mamba T. S.
  • Detection of Entamoeba histolytica by Recombinase Polymerase Amplification. Am. J. Trop. Med. 2015; 93 (3) 591-595 (2015)
    Nair G. et al.
  • Detection of Helminth Ova in Wastewater Using Recombinase Polymerase Amplification Coupled to Lateral Flow Strips. (2020)
    Ravindran V. B., Khallaf B., Surapaneni A., Crosbie N.D., Soni S.K., Ball A.S.
  • Detection of Infectious Viruses Using CRISPR-Cas12-Based Assay (2021)
    Talwar, C. S., Park, K. H., Ahn, W. C., Kim, Y. S., Kwon, O. S., Yong, D., Kang, T., & Woo, E.
  • Detection of Mycobacterium tuberculosis by using loop-mediated isothermal amplification combined with a lateral flow dipstick in clinical samples. Biomed. Res. Int. 2013; 1-6 (2013)
    Kaewphinit T., Arunrut N., Kiatpathomchai W., Santiwatanakul S., Jaratsing P., Chansiri K.
  • Detection of Reverse Transcriptase LAMP-Amplified Nucleic Acid from Oropharyngeal Viral Swab Samples Using Biotinylated DNA Probes through a Lateral Flow Assay (2023)
    Agarwal, S., Hamidizadeh, M., & Bier, F. F.
  • Detection of SARS-CoV-2 Using Reverse Transcription Helicase Dependent Amplification and Reverse Transcription Loop-Mediated Amplification Combined with Lateral Flow Assay (2022)
    Zasada, A. A., Mosiej, E., Prygiel, M., Polak, M., Wdowiak, K., Formińska, K., Ziółkowski, R., Żukowski, K., Marchlewicz, K., Nowiński, A., Nowińska, J., Rastawicki, W., & Malinowska, E.
  • Detection of Skeletonema costatum based on loop-mediated isothermal amplification combined with lateral flow dipstick. Mol Cell Probes. 2017 Dec;36:36-42. (2017)
    Huang H. L. et al.
  • Developing a surface acoustic wave-induced microfluidic cell lysis device for point-of-care DNA amplification (2023)
    Husseini, A. A., Yazdani, A. M., Ghadiri, F., & Şişman, A.
  • Development and application of sensitive, specific, and rapid CRISPR-Cas13-based diagnosis (2021)
    Shihong Gao, D., Zhu, X., & Lu, B.
  • Development and Characterization of Recombinase-Based Isothermal Amplification Assays (RPA/RAA) for the Rapid Detection of Monkeypox Virus (2022)
    Mao, L., Ying, J., Selekon, B., Gonofio, E., Wang, X., Nakoune, E., Wong, G., & Berthet, N.
  • Development and Clinical Validation of RT-LAMP-Based Lateral-Flow Devices and Electrochemical Sensor for Detecting Multigene Targets in SARS-CoV-2 (2022)
    Canuti, M., Saxena, A., Rai, P., Mehrotra, S., Baby, S., Singh, S., Srivastava, V., Priya, S., & Sharma, S. K.
  • Development and evaluation of a novel visual and rapid detection assay for toxigenic Fusarium graminearum in maize based on recombinase polymerase amplification and lateral flow analysis (2022)
    Liang, X., Zhang, X., Haseeb, H. A., Tang, T., Shan, J., Yin, B., & Guo, W.
  • Development and evaluation of an isothermal recombinase polymerase amplification–lateral flow assay for rapid detection of strawberry vein banding virus in the field (2022)
    Zou, X., Yuan, S., Dong, C., & Gao, Q.
  • Development and evaluation of serotype-specific recombinase polymerase amplification combined with lateral flow dipstick assays for the diagnosis of foot-and-mouth disease virus serotype A, O and Asia1 (2018)
    Wang H, Hou P, Zhao G, Yu L, Gao YW, He H
  • Development and pre-clinical evaluation of a Zika virus diagnostic for low resource settings (2023)
    Balea, R., Pollak, N. M., Hobson-Peters, J., Macdonald, J., & McMillan, D. J.
  • Development of a duplex lateral flow dipstick test for the detection and differentiation of Listeria spp. and Listeria monocytogenes in meat products based on loop-mediated isothermal amplification (2019)
    Ledloda S, Bunroddithd K, Areekit S, Santiwatanakulc S, Chansiria K
  • Development of a lateral flow recombinase polymerase amplification assay for rapid and visual detection of Cryptococcus neoformans/C. gattii in cerebral spinal fluid (2019)
    Ma Q, Yao J, Yuan S, Liu H, Wei N, Zhang J, Shan W
  • Development of a Loop-Mediated Isothermal Amplification Assay Coupled With a Lateral Flow Dipstick Test for Detection of Myosin Binding Protein C3 A31P Mutation in Maine Coon Cats (2022)
    Sukumolanan, P., Demeekul, K., & Petchdee, S.
  • Development of a Rapid and Sensitive CasRx-Based Diagnostic Assay for SARS-CoV-2 (2021)
    Brogan, D. J., Chaverra-Rodriguez, D., Lin, C. P., Smidler, A. L., Yang, T., Alcantara, L. M., Antoshechkin, I., Liu, J., Raban, R. R., Belda-Ferre, P., Knight, R., Komives, E. A., & Akbari, O. S.
  • Development of a Rapid and Sensitive CasRx-Based Diagnostic Assay for SARS-CoV-2 (2022)
    Brogan, D. J., Chaverra-Rodriguez, D., Lin, C. P., Smidler, A. L., Yang, T., Alcantara, L. M., Antoshechkin, I., Liu, J., Raban, R. R., Belda-Ferre, P., Knight, R., Komives, E. A., & Akbari, O. S.
  • Development of a rapid and visual detection method for Rickettsia rickettsii combining recombinase polymerase assay with lateral flow test (2018)
    Qi Y, Shao Y, Rao J, Shen W, Yin Q, Li X, Chen H, Li J, Zeng W, Zheng S, Liu S, Li Y
  • Development of a rapid and visual nucleotide detection method for a Chinese epidemic strain of Orientia tsutsugamushi based on recombinase polymerase amplification assay and lateral flow test (2018)
    Qia Y, Yin Q, Shaob Y, Caoa M, Lia S, Chena H, Shena W, Raoa J, Lia J, Lib X, Sunc Y, Linb Y, Dengb Y, Zengb W, Zhengb S, Liub S, Lia Y
  • Development of a rapid detection method for Karenia mikimotoi by using CRISPR-Cas12a (2023)
    Wang, L., Chen, X., Pan, F., Yao, G., & Chen, J.
  • Development of a rapid PCR-nucleic acid lateral flow immunoassay (PCR-NALFIA) based on rDNA IGS sequence analysis for the detection of Macrophomina phaseolina in soil (2018)
    Pecchia S, Da Lio D
  • Development of a Rapid Reverse Transcription-Recombinase Polymerase Amplification Couple Nucleic Acid Lateral Flow Method for Detecting Porcine Epidemic Diarrhoea Virus (2022)
    Desselberger, U., Davidson, A., Pewlaoo, S., Phanthong, S., Kong-Ngoen, T., Santajit, S., Tunyong, W., Buranasinsup, S., Kaeoket, K., Thavorasak, T., Pumirat, P., Sookrung, N., Chaicumpa, W., & Indrawattana, N.
  • Development of a Rapid Sex Identification Method for Newborn Pigeons Using Recombinase Polymerase Amplification and a Lateral-Flow Dipstick on Farm (2022)
    Lai, F.-Y. ;, Chang, K.-C. ;, Chang, C.-S. ;, Wang, P.-H., Lai, F.-Y., Chang, K.-C., Chang, C.-S., & Wang, P.-H.
  • Development of a Recombinase Polymerase Amplification Assay for Schistosomiasis Japonica Diagnosis in the Experimental Mice and Domestic Goats (2021)
    Guo, Q., Zhou, K., Chen, C., Yue, Y., Shang, Z., Zhou, K., Fu, Z., Liu, J., Lin, J., Xia, C., Tang, W., Cong, X., Sun, X., & Hong, Y.
  • Development of a recombinase polymerase amplification combined with a lateral flow dipstick assay for rapid detection of the Mycoplasma bovis (2018)
    Zhao G, Hou P, Huan Y, He C, Wang H, He H
  • Development of a Recombinase Polymerase Amplification Combined with Lateral Flow Dipstick Assay for Rapid and Sensitive Detection of Bean Common Mosaic Virus (2021)
    Qin, J., Yin, Z., Shen, D., Chen, H., Chen, X., Cui, X., Chen, H.
  • Development of a recombinase polymerase amplification lateral flow assay for the detection of active Trypanosoma evansi infections. (2020)
    Li Z., Torres J., Goossens J., et. al.
  • Development of a reverse transcription recombinase polymerase amplification assay for rapid and direct visual detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) (2021)
    Lau, Y. L., Ismail, I. B., Mustapa, N., Lai, M. Y., Tuan Soh, T. S., Haji Hassan, A., Peariasamy, K. M., Lee, Y. L., Abdul Kahar, M., Chong, J., & Goh, P. P.
  • Development of a reverse transcription recombinase polymerase amplification combined with lateral flow assay for equipment-free on-site field detection of tomato chlorotic spot virus (2023)
    Yilmaz, S., & Batuman, O.
  • Development of an Isothermal Amplification-Based Assay for Rapid Visual Detection of an Orf Virus. Virol. J. 2016; 13:46 (2016)
    Yang Y et al.
  • Development of an isothermal nucleic acid amplification protocol for high-throughput monitoring of Plum pox virus infection in stone fruit production (2012)
    Hadersdorfer J.
  • Development of CRISPR/Cas13a-based assays for the diagnosis of Schistosomiasis (2023)
    MacGregor, S. R., McManus, D. P., Sivakumaran, H., Egwang, T. G., Adriko, M., Cai, P., Gordon, C. A., Duke, M. G., French, J. D., Collinson, N., Olveda, R. M., Hartel, G., Graeff-Teixeira, C., Jones, M. K., & You, H.
  • Development of cross-priming amplification (CPA) combined with colorimetric and lateral flow dipstick visualization for scale drop disease virus (SDDV) detection (2021)
    Prasitporn, T., Senapin, S., Vaniksampanna, A., Longyant, S., & Chaivisuthangkura, P.
  • Development of Recombinase Polymerase Amplification Assays for Detection of Orienta Tsutsugamushi or Rickettsia typhi. PLoS Negl. Trop. Dis. 2015; 9 (7) (2015)
    Chao CC et al.
  • Development of recombinase polymerase amplification combined with lateral flow dipstick assay to detect hemolysin gene of Vibrio vulnificus in oysters (2022)
    Park, S., & Chang, S.
  • Development of simple, rapid, and sensitive methods for detection of hepatitis C virus RNA from whole blood using reverse transcription loop-mediated isothermal amplification (2023)
    Pauly, M. D., Weis-Torres, S., Hayden, T. M., Ganova-Raeva, L. M., & Kamili, S.
  • Development of the Rapid Test Kit for the Identification of Campylobacter spp. Based on Loop-mediated Isothermal Amplification (LAMP) in Combination with a Lateral Flow Dipstick (LFD) and Gold Nano-DNA Probe (AuNPs) (2019)
    Thongphueak D, Chansiri K, Sriyapai T , Areekit S, Santiwatanakul S, Wangroongsarb P
  • Diagnostics of COVID-19 Based on CRISPR-Cas Coupled to Isothermal Amplification: A Comparative Analysis and Update (2022)
    Diagnostics, A., Hernandez-Garcia, A., Morales-Moreno, M. D., Valdés-Galindo, E. G., Jimenez-Nieto, E. P., & Quezada, A.
  • Diagnostics of Infections Produced by the Plant Viruses TMV, TEV, and PVX with CRISPR-Cas12 and CRISPR-Cas13 (2022)
    Marqués, M.-C., Sánchez-Vicente, J., Ruiz, R., Montagud-Martínez, R., Márquez-Costa, R., Gómez, G., Carbonell, A., Daròs, J.-A., & Rodrigo, G.
  • Disposable and low-cost pen-like sensor incorporating nucleic-acid amplification based lateral-flow assay for at-home of communicable pathogens (2022)
    Lu, X., Lin, H., Feng, X., Lui, G. CY., & Hsing, I.-M.
  • DNA Detectin Using Recombination Proteins (2006)
    Piepenburg O., Williams C.H., Stemple D.L., Armes N.A.
  • Dual Methylation-Sensitive Restriction Endonucleases Coupling with an RPA-Assisted CRISPR/Cas13a System (DESCS) for Highly Sensitive Analysis of DNA Methylation and Its Application for Point-of-Care Detection (2021)
    Wang, X., Zhou, S., Chu, C., Yang, M., Huo, D., & Hou, C.
  • Electricity-free amplification and detection for molecular point-of-care diagnosis of HIV-1 (2014)
    Singleton, J., Osborn, J. L., Lillis, L., Hawkins, K., Guelig, D., , W., Johns, R., Ebels, K., Boyle, D., Weigl, B., & LaBarre, P.
  • Endonuclease-Assisted PAM-free Recombinase Polymerase Amplification Coupling with CRISPR/Cas12a (E-PfRPA/Cas) for Sensitive Detection of DNA Methylation (2022)
    Zhou, S., Dong, J., Deng, L., Wang, G., Yang, M., Wang, Y., Huo, D., & Hou, C.
  • Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection. (2020)
    Nguyen L., Smith B., Jain P
  • Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection. (2020)
    Nguyen L., Smith B., Jain P.
  • Equipment-free detection of SARS-CoV-2 and Variants of Concern using Cas13 (2021)
    Arizti-Sanz, J., Bradley, A. D., Zhang, Y. B., Boehm, C. K., Freije, C. A., Grunberg, M. E., Kosoko-Thoroddsen, T. F., Welch, N. L., Pillai, P. P., Mantena, S., Kim, G., Uwanibe, J. N., John, O. G., Eromon, P. E., Kocher, G., Gross, R., Lee, J. S., Hensley, L. E., Happi, C. T., Johnson, J., Myhrvold, C.
  • Establishment and application of the Recombinase-Aided Amplification-Lateral Flow Dipstick detection method for Pantoea ananatis on rice (2023)
    Aiying, W., Ju, L., Cilin, W., Yuxuan, H., Baojun, Y., Jian, T., & Shuhua, L.
  • Establishment of methods for rapid detection of Prymnesium parvum by recombinase polymerase amplification combined with a lateral flow dipstick (2022)
    Luo, N., Huang, H., & Jiang, H.
  • Establishment of the recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) detection technique for Fusarium oxysporum (2023)
    Hu, M. S., Yan, M. C., Yu, Prof. H., Zhang, Dr. Y., & Zhang, Mr. C.
  • Evaluation of CRISPR/Cas12a-based DNA detection for fast pathogen diagnosis and GMO test in rice. (2020)
    Zhang Y., Xie K.,
  • Evaluation of diagnostic methods for vector-borne viral infections and development of tools for the study of yellow fever infections (2014)
    Escadafal C.
  • Fast and sensitive detection of SARS-CoV-2 RNA using suboptimal protospacer adjacent motifs for Cas12a (2022)
    Lu, S., Tong, X., Han, Y., Zhang, K., Zhang, Y., Chen, Q., Duan, J., Lei, X., Huang, M., Qiu, Y., Zhang, D. Y., Zhou, X., Zhang, Y., & Yin, H.
  • Feasibility of a DNA biosensor assay based on loop-mediated isothermal amplification combined with a lateral flow dipstick assay for the visual detection of Ascaridia galli eggs in faecal samples (2023)
    Panich, W., Tejangkura, T., & Chontananarth, T.
  • Field-deployable assay based on CRISPR-Cas13a coupled with RT-RPA in one tube for the detection of SARS-CoV-2 in wastewater (2023)
    Yang, Y., Wang, F., Xue, B., & Zhou, X.
  • FnCas9 Editor Linked Uniform Detection Assay for COVID-19 (2022)
    Phutela, R., Gulati, S., Kumar, M., Maiti, S., & Chakraborty, D.
  • FnCas9-based CRISPR diagnostic for rapid and accurate detection of major SARS-CoV-2 variants on a paper strip (2021)
    Kumar, M., Gulati, S., Ansari, A. H., Phutela, R., Acharya, S., Azhar, M., Murthy, J., Kathpalia, P., Kanakan, A., Maurya, R., Vasudevan, J. S., S, A., Pandey, R., Maiti, S., & Chakraborty, D.
  • G-triplex: A new type of CRISPR-Cas12a reporter enabling highly sensitive nucleic acid detection (2021)
    Li, T., Hu, R., Xia, J., Xu, Z., Chen, D., Xi, J., Liu, B. F., Zhu, J., Li, Y., Yang, Y., & Liu, M.
  • Genetic identification of Staphylococcus aureus isolates from cultured milk samples of bovine mastitis using isothermal amplification with CRISPR/Cas12a-based molecular assay (2023)
    Amanzholova, M., Shaizadinova, A., Bulashev, A., & Abeldenov, S.
  • Genome-informed diagnostics for specific and rapid detection of Pectobacterium species using recombinase polymerase amplification coupled with a lateral flow device (2018)
    Ahmed FA, Larrea-Sarmiento A, Alvarez AM, Arif M
  • Genome-wide tiled detection of circulating Mycobacterium tuberculosis cell-free DNA using Cas13 (2023)
    Thakku, S. G., Lirette, J., Murugesan, K., Chen, J., Theron, G., Banaei, N., Blainey, P. C., Gomez, J., Wong, S. Y., & Hung, D. T.
  • High-Surety Isothermal Amplification and Detection of SARS-CoV-2 (2021)
    Bhadra, S., Riedel, T. E., Lakhotia, S., Tran, N. D., & Ellington, A. D.
  • Highly Specific and Rapid Detection of Hepatitis C Virus Using RT-LAMP-Coupled CRISPR-Cas12 Assay (2022)
    Kham-Kjing, N., Ngo-Giang-Huong, N., Tragoolpua, K., Khamduang, W., & Hongjaisee, S.
  • Highly specific and sensitive detection of Burkholderia pseudomallei genomic DNA by CRISPR-Cas12a (2022)
    Pop, S., Id, W., Thananchai, H., Chewapreecha, C., Roslund, H. B., Chomkatekaew, C., Tananupak, W., Boonklang, P., Pakdeerat, S., Seng, R., Chantratita, N., Takarn, P., & Khamnoi, P.
  • Identification of a New Badnavirus in the Chinaberry (Melia azedarach) Tree and Establishment of a LAMP-LFD Assay for Its Rapid and Visual Detection (2021)
    Lu, H., Tang, J., Sun, K., & Yu, X.
  • Inhibiotion of Recombinase Polymerase Amplification by Background DNA: A Lateral Flow-Based Method for Enriching Target DNA. Anal. Chem. 2015 87 (3), 1963-1967 (2015)
    Rohrman B. et al.
  • Instrument-free, CRISPR-based diagnostics of SARS-CoV-2 using self-contained microfluidic system (2021)
    Li, Z., Ding, X., Yin, K., Avery, L., Ballesteros, E., & Liu, C.
  • Integration of CRISPR/Cas12a and Multiplexed RPA for Fast Detection of Gene Doping (2022)
    Yan, J., Xu, Z., Zhou, H., Li, T., Du, X., Hu, R., Zhu, J., Ou, G., Li, Y., & Yang, Y.
  • Isothermal amplification-mediated lateral flow biosensors for in vitro diagnosis of gastric cancer-related microRNAs (2022)
    Seo, S. B., Hwang, J. S., Kim, E., Kim, K., Roh, S., Lee, G., Lim, J., Kang, B., Jang, S., Son, S. U., Kang, T., Jung, J., Kim, J. S., Keun-Hur, Han, T. S., & Lim, E. K.
  • Isothermal DNA amplification combined with lateral flow dipsticks for detection of biothreat agents. Anal Biochem. 2018 Nov 1;560:60-66. (2018)
    Zasada A. A. et al.
  • Isothermal Recombinase Polymerase Amplification (RPA) of Schistosoma haematobium DNA and Oligochromatographic Lateral Flow Detection. Parasit Vectors 2015; 8 (2015)
    Rosser et al.
  • Lateral Flow Loop-Mediated Isothermal Amplification Test with Stem Primers: Detection of Cryptosporidium Species in Kenyan Children Presenting with Diarrhea. J Trop Med. 2018 Feb 26;2018:7659730 (2018)
    Mamba T. S. et al.
  • Lateral flow–based nucleic acid detection of SARS-CoV-2 using enzymatic incorporation of biotin-labeled dUTP for POCT use (2022)
    Agarwal, S., Warmt, C., Henkel, J., Schrick, L., Nitsche, A., & Bier, F. F.
  • Loop-mediated isothermal amplification (LAMP)-based method for rapid mushroom species identification. J. Agric. Food Chem. 2013; 61 (8), 1833-1840. (2013)
    Vaagt F et al.
  • Loop-mediated isothermal amplification (LAMP)/Cas12a assay for detection of Ralstonia solanacearum in tomato (2023)
    Fan, Z., Mei, Y., Xing, J., Chen, T., Hu, D., Liu, H., Li, Y., Liu, D., Liu, Z., & Liang, Y.
  • Loop-mediated isothermal amplification assay for fluorescence analysis and lateral flow detection of male DNA (2022)
    Kubo, S., Niimi, H., & Kitajima, I.
  • Loop-Mediated Isothermal Amplification-Lateral-Flow Dipstick (LAMP-LFD) to detect Toxoplasma gondii oocyst in ready-to-eat salad (2018)
    Lalle M, Possenti A, P. Dubey JP, Pozio E
  • Metagenomic sequencing for identifying pathogen-specific circulating DNAs and development of diagnostic methods for schistosomiasis (2023)
    Liu, J., Wang, X., Sheng, F., Giri, B. R., Li, S., Xia, T., Li, X., & Cheng, G.
  • Methods for the detection of allergens in wine (2017)
    Wessels H. T.
  • Microscopic and LF-RPA assay approaches to the detection of the fungal peanut pathogen Cercospora arachidicola (2022)
    Lin, Y., Lu, X., Liu, X., Xie, J., Pei, X., Yu, S., Zang, C., & Liang, C.
  • Molecular method for rapid detection of the red tide dinoflagellate Karenia mikimotoi in the coastal region of Xiangshan Bay, China. (2020)
    Huang H. L., Gao W.F., Zhu P., Zhou C.X., Qiao L.L., Dang C.Y., Pang J.H., Yan X.J.,
  • Monitoring Pathogenic Viable E. coli O157:H7 in Food Matrices Based on the Detection of RNA Using Isothermal Amplification and a Paper-Based Platform (2021)
    Petrucci, S., Costa, C., Broyles, D., Kaur, A., Dikici, E., Daunert, S., & Deo, S. K.
  • Multi-detection for Paramphistomes Using Novel Manually Designed PAR-LAMP Primers and its Application in a Lateral Flow Dipstick (LAMP-LFD) System (2023)
    Nak-on, S., Tejangkura, T., & Chontananarth, T.
  • Multicenter Testing of a Simple Molecular Diagnostic System for the Diagnosis of Mycobacterium Tuberculosis (2023)
    Lee, H. J., Kim, N. H., Lee, E. H., Yoon, Y. S., Jeong, Y. J., Lee, B. C., Koo, B., Jang, Y. O., Kim, S.-H., Kang, Y. A., Lee, S. W., & Shin, Y.
  • New design strategies for ultra-specific CRISPR-Cas13a-based RNA detection with single-nucleotide mismatch sensitivity (2023)
    Molina Vargas, A. M., Sinha, S., Osborn, R., Arantes, P. R., Patel, A., Dewhurst, S., Hardy, D. J., Cameron, A., Palermo, G., & O’Connell, M. R.
  • NEXT CRISPR: An enhanced CRISPR-based nucleic acid biosensing platform using extended crRNA (2022)
    Ganbaatar, U., & Liu, C.
  • Novel high-performance detection of Raillietina echinobothrida, Raillietina tetragona, and Raillietina cesticillus using loop-mediated isothermal amplification coupled with a lateral flow dipstick (LAMP-LFD) (2021)
    Panich, W., Tejangkura, T., & Chontananarth, T.
  • Nucleic acid detection of plant genes using CRISPR-Cas13 (2019)
    Abudayyeh OO, Gootenberg JS, Kellner MJ, Zhang F
  • Nucleic Acid Detection Using CRISPR/Cas Biosensing Technologies. (2020)
    Aman R., Mahas A., Mahfouz M.,
  • Nucleic Acid Lateral Flow Immunoassays: Nachweisentwicklung, Limitationen und methodische Neuerungen (2021)
    Breitbach, A.
  • Obtaining Specific Sequence Tags for Yersinia pestis and Visually Detecting Them Using the CRISPR-Cas12a System (2021)
    Chen, G., Lyu, Y., Wang, D., Zhu, L., Cao, S., Pan, C., Feng, E., Zhang, W., Liu, X., Cui, Y., & Wang, H.
  • One-Step Reverse-Transcription Recombinase Polymerase Amplification Using Lateral Flow Strips for the Detection of Coxsackievirus A6 (2021)
    Xie, J., Yang, X., Duan, L., Chen, K., Liu, P., Zhan, W., Zhang, C., Zhao, H., Wei, M., Tang, Y., & Luo, M.
  • Optimization of the Isothermal Amplification Method for Mycobacterium Tuberculosis Detection and Visualization for Fieldwork. (2020)
    Ağel H.E., Sağcan H., Ceyhan I., Durmaz R.
  • Paper Device Combining CRISPR/Cas12a and Reverse-Transcription Loop-Mediated Isothermal Amplification for SARS-CoV-2 Detection in Wastewater (2022)
    Cao, H., Mao, K., Ran, F., Xu, P., Zhao, Y., Zhang, X., Zhou, H., Yang, Z., Zhang, H., & Jiang, G.
  • PNA-Pdx: Versatile Peptide Nucleic Acid-Based Detection of Nucleic Acids and SNPs (2023)
    Jiang, W., Aman, R., Ali, Z., Rao, G. S., & Mahfouz, M.
  • Point of care colourimetric and lateral flow LAMP assay for the detection of Haemonchus contortus in ruminant faecal samples (2021)
    Khangembam, R., Tóth, M., Vass, N., Várady, M., Czeglédi, L., Farkas, R., & Antonopoulos, A.
  • Point-of-care detection of Neisseria gonorrhoeae based on RPA-CRISPR/Cas12a (2023)
    Tu, Q., Cao, X., Ling, C., Xiang, L., Yang, P., & Huang, S.
  • Point-of-care testing for COVID-19 using SHERLOCK diagnostics. (2020)
    Joung J., Ladha A., Saito M., et. al.
  • Polyethersulfone-Based Microfluidic Device Integrated with DNA Extraction on Paper and Recombinase Polymerase Amplification for the Detection of Salmonella enterica (2023)
    Chen, Y., Hu, Y., & Lu, X.
  • Pseudo-Complementary G:C Base Pair for Mixed Sequence dsDNA Invasion and Its Applications in Diagnostics (2023)
    López-Tena, M., Farrera-Soler, L., Barluenga, S., & Winssinger, N.
  • Quick hassle-free detection of cyprinid herpesvirus 2 (CyHV-2) in goldfish using recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) assay (2022)
    Preena, P. G., Kumar, T. V. A., Johny, T. K., Dharmaratnam, A., & Swaminathan, T. R.
  • Rapid analysis of Escherichia coli O157:H7 using isothermal recombinase polymerase amplification combined with triple-labeled nucleotide probes. (2020)
    Hu J., Wang Y., Su H., et. al.
  • Rapid and accurate detection of novel coronavirusSARS-CoV-2 using CRISPR-Cas3. (2020)
    Yoshimi K., Takeshita K., Yamayoshi S., et. al.
  • Rapid and accurate nucleobase detection using FnCas9 and its application in COVID-19 diagnosis (2021)
    Azhar, M., Phutela, R., Kumar, M., Ansari, A. H., Rauthan, R., Gulati, S., Sharma, N., Sinha, D., Sharma, S., Singh, S., Acharya, S., Sarkar, S., Paul, D., Kathpalia, P., Aich, M., Sehgal, P., Ranjan, G., Bhoyar, R. C.
  • Rapid and accurate nucleobase detection using FnCas9 and its application in COVID-19 diagnosis (2021)
    Azhar, M., Phutela, R., Kumar, M., Ansari, A. H., Rauthan, R., Gulati, S., Sharma, N., Sinha, D., Sharma, S., Singh, S., Acharya, S., Sarkar, S., Paul, D., Kathpalia, P., Aich, M., Sehgal, P., Ranjan, G., Bhoyar, R. C., Singhal, K., … Maiti, S.
  • Rapid and accurate species identification for ecological studies and monitoring using CRISPR-based SHERLOCK. (2020)
    Baerwald M., Goodbla A., nagarajan R., Gootenberg J., Abudayyeh O., Zhang F., Schreier A.
  • Rapid and highly sensitive LAMP-CRISPR/Cas12a-based identification of bovine mastitis milk samples contaminated by Escherichia coli (2023)
    Shaizadinova, A., Amanzholova, M., Kirillov, S., Bulashev, A., & Abeldenov, S.
  • Rapid and sensitive detection of Babesia bovis and Babesia bigemina by loop-mediated isothermal amplification combined with a lateral flow dipstick Vet. Parasitol 2016; 219, 71-76 (2016)
    Yang Y et al.
  • Rapid and sensitive detection of Candidatus Liberibacter asiaticus by loop mediated isothermal amplification combined with a lateral flow dipstick BMC Microbiol. 2014; 1186 (2014)
    Rigano AL et al.
  • Rapid and sensitive detection of infectious spleen and kidney necrosis virus by loop-mediated isothermal amplification combined with a lateral flow dipstick (2010)
    W.C. Ding, Jiong Chen, Y.H. Shi, X.J. Lu, M.Y. Li
  • Rapid and sensitive detection of infectious spleen and kidney necrosis virus by recombinase polymerase amplification combined with lateral flow dipsticks. (2020)
    Li H., Yuan G., Luo Y., Yu Y., Ai T., Su J.
  • Rapid and sensitive detection of Karenia mikimotoi by loop-mediated isothermal amplification combined with a lateral flow dipstick (2021)
    Han, X., Zhao, T., Yan, T., & Yu, R.
  • Rapid and sensitive detection of Little cherry virus 2 using isothermal reverse transcription-recombinase polymerase amplification (2014)
    Mekuria, T. A., Zhang, S., & Eastwell, K. C.
  • Rapid and sensitive detection of pathogenic Elizabethkingia miricola in black spotted frog by RPA-LFD and fluorescent probe-based RPA (2022)
    Qiao, M., Zhang, L., Chang, J., Li, H., Li, J., Wang, W., Yuan, G., & Su, J.
  • Rapid and simple detection of Glaesserella parasuis in synovial fluid by recombinase polymerase amplification and lateral flow strip (2019)
    Zhang TT, Liu MZ, Yin RH, Yao LQ, Liu BS, Chen ZL
  • Rapid and simple detection of Phytophthora cactorum in strawberry using a coupled recombinase polymerase amplification-lateral flow strip assay (2021)
    Lu, X., Xu, H., Song, W., Yang, Z., Yu, J., Tian, Y., Jiang, M., Shen, D., & Dou, D.
  • Rapid and Visual Detection of Barley Yellow Dwarf Virus by Reverse Transcription Recombinase Polymerase Amplification with Lateral Flow Strips (2022)
    Kim, N. K., Lee, H. J., Kim, S. M., & Jeong, R. D.
  • Rapid and visual detection of enterovirus using recombinase polymerase amplification combined with lateral flow strips. (2020)
    Yang X., Xie J., Hu S., Zhan W., Duan L., Chen K., Zhang C., Yin A., Luo M
  • Rapid and Visual Detection of Heterodera schachtii Using Recombinase Polymerase Amplification Combined with Cas12a-Mediated Technology (2021)
    Yao, K., Peng, D., Jiang, C., Zhao, W., Li, G., Huang, W., Kong, L., Gao, H., Zheng, J., & Peng, H.
  • Rapid and visual detection of Mycoplasma synoviae by recombinase-aided amplification assay combined with a lateral flow dipstick (2022)
    Xia, W., Chen, K., Liu, W., Yin, Y., Yao, Q., Ban, Y., Pu, Y., Zhan, X., Bian, H., Yu, S., Han, K., Yang, L., Wang, H., & Fan, Z.
  • Rapid and visual detection of porcine deltacoronavirus by recombinase polymerase amplification combined with a lateral flow dipstick (2020)
    Gao X., Liu X., Zhang Y., et. al.
  • Rapid and visual detection of tomato spotted wilt virus using recombinase polymerase amplification combined with lateral flow strips (2021)
    Lee, H. J., Cho, I. S., Ju, H. J., & Jeong, R. D.
  • Rapid and visual detection of Trichinella spp. using a lateral flow strip-based recombinase polymerase amplification (LF-RPA) assay (2019)
    Li TT, Wang JL, Zhang NZ, Li WH, Yan HB, Li L,Jia WZ, Fu BQ
  • Rapid and visual detection of Verticillium dahliae using recombinase polymerase amplification combined with lateral flow dipstick. (2020)
    Ju Y., Li C., Shen P., et. al.,
  • Rapid authentication of Cordyceps by lateral flow dipstick. J. Pharm. Biomed. Anal 2015, 111, 306-310 (2015)
    Wong YL et al.
  • Rapid detection and differentiation of carp oedema virus and cyprinid herpes virus-3 in koi and common carp. J Fish Dis. 2018 May;41(5):761-772. (2018)
    Soliman H. and El-Matoubli M.
  • Rapid Detection of 2019 Novel Coronavirus SARS-CoV-2 Using a CRISPR-based DETECTR Lateral Flow Assay. (2020)
    Broughton J. P., Deng X., Fasching C.L., Singh J., Streithorst J., Granados, A., Sotomayor-Gonzalez A., Zorn K., Gopez A., Hsu E., Gu W., Miller S., Pan C., Guevara H., Wadford D., Chen J., Chio C.Y.
  • Rapid detection of African swine fever virus using Cas12a-based portable paper diagnostics. (2020)
    Lu L., Li F., Chen Q., et. al
  • Rapid detection of bovine viral diarrhea virus using recombinase polymerase amplification combined with lateral flow dipstick assays in bulk milk (2018)
    Hou P, Zhao G, Wang H, He C, He H
  • Rapid detection of Burkholderia pseudomallei with a lateral flow recombinase polymerase amplification assay (2019)
    Peng Y, Zheng X, Kann B, Li W, Zhang W, Jiang T, Lu J, Qin A
  • Rapid detection of Edwardsiella ictaluri in yellow catfish (Pelteobagrus fulvidraco) by real-time RPA and RPA-LFD (2022)
    Li, H., Zhang, L., Yu, Y., Ai, T., Zhang, Y., & Su, J.
  • Rapid detection of Enterococcus and vancomycin resistance using recombinase polymerase amplification (2021)
    Panpru, P., Srisrattakarn, A., Panthasri, N., Tippayawat, P., Chanawong, A., Tavichakorntrakool, R., Daduang, J., Wonglakorn, L., & Lulitanond, A.
  • Rapid detection of HIV-1 proviral DNA for early infant diagnosis using recombinase polymerase amplification (2013)
    Boyle, D. S., Lehman, D. A., Lillis, L., Peterson, D., Singhal, M., Armes, N., Parker, M., Piepenburg, O., & Overbaugh, J.
  • Rapid detection of kdr mutation F1534C in Aedes aegypti using recombinase polymerase amplification and lateral flow dipsticks (2022)
    Ahmed, M., Nath, N. S., Hugo, L. E., Devine, G. J., Macdonald, J., & Pollak, N. M.
  • Rapid detection of mcr-1 by recombinase polymerase amplification (2018)
    Xu, J., Wang, X., Yang, L., Kan, B., & Lu, X.
  • Rapid detection of mcyG gene of microcystins producing cyanobacteria in water samples by recombinase polymerase amplification combined with lateral flow strips (2021)
    Li, J., Feng, M., & Yu, X.
  • Rapid detection of methicillin-resistant Staphylococcus aureus in positive blood-cultures by recombinase polymerase amplification combined with lateral flow strip (2022)
    Srisrattakarn, A., Panpru, P., Tippayawat, P., Chanawong, A., Tavichakorntrakool, R., Daduang, J., Wonglakorn, L., & Id, A. L.
  • Rapid detection of monkeypox virus using a CRISPR-Cas12a mediated assay: a laboratory validation and evaluation study (2023)
    Caly, L., Pasricha, S., Williamson, D. A., Kerry MBiotech, W. J., Whitehead, L. W., Williams, L., Cooney, J. P., Pellegrini, M., Savic, I., Prestedge, J., Tran, T., Lim, C. K., Towns, J. M., Bradshaw, C. S., Fairley, C., F Chow, E. P., Chen, M. Y., Jen Low, S., O, M. T., … Williamson, D. A.
  • Rapid Detection of SARS-CoV-2 RNA Using Reverse Transcription Recombinase Polymerase Amplification (RT-RPA) with Lateral Flow for N-Protein Gene and Variant-Specific Deletion–Insertion Mutation in S-Protein Gene (2023)
    Tanuri, A., Malaga, J. L., Pajuelo, M. J., Okamoto, M., Kagning Tsinda, E., Otani, K., Tsukayama, P., Mascaro, L., Cuicapuza, D., Katsumi, M., Kawamura, K., Nishimura, H., Sakagami, A., Ueki, Y., Omiya, S., Okamoto, S., Nakayama, A., Fujimaki, S., Yu, C., … Saito, M.
  • Rapid Detection of Strawberry Mild Yellow Edge Virus with a Lateral Flow Strip Reverse Transcription Recombinase Polymerase Amplification Assay (2022)
    Zou, X., Dong, C., Ni, Y., & Gao, Q.
  • Rapid detection of strawberry mottle virus using reverse transcription recombinase polymerase amplification with lateral flow strip (2022)
    Zou, X., Dong, C., Ni, Y., Yuan, S., & Gao, Q. H.
  • Rapid detection of the rice false smut fungus Ustilaginoidea virens by lateral flow strip-based recombinase polymerase amplification assay (2023)
    Jia-cheng, X., San-lian, W., Yue, L., Yuan-di, X., Jing, Y., Ting, Z., Jia-jia, C., Zheng-guang, Z., Dan-yu, S., & Hai-feng, Z.
  • Rapid Detection of the Varicella-Zoster Virus Using a Recombinase-Aided Amplification-Lateral Flow System (2022)
    Bienes, K. M., Mao, L., Selekon, B., Gonofio, E., Nakoune, E., Wong, G., & Berthet, N.
  • Rapid Diagnosis and Visual Detection of Potato Cyst Nematode (Globodera rostochiensis) Using Recombinase Polymerase Amplification Combination with Lateral Flow Assay Method (RPA-LFA) (2022)
    Wang, X. ;, Lei, R. ;, Peng, H. ;, Jiang, R. ;, Shao, H. D. ;, Peng, J. J. ;, Wang, X., Lei, R., Peng, H., Jiang, R., Shao, H., Ge, J., & Peng, D.
  • Rapid diagnosis of two marine viruses, red sea bream iridovirus and viral hemorrhagic septicemia virus by PCR combined with lateral flow assay. (2020)
    Seo H., Kil E., Fadhila C., et. al.
  • Rapid Diagnostic for the Detection of the Four Dengue Virus Serotypes in Clinically Relevant Matrices (2023)
    Pollak, N. M., Olsson, M., Ahmed, M., Tan, J., Lim, G., Setoh, Y. X., Wong, J. C. C., Lai, Y. L., Hobson-Peters, J., Macdonald, J., & McMillan, D.
  • Rapid DNA extraction and colorimetric amplicon visualisation speed up LAMP-based detection of soybean allergen in foods (2023)
    Schäfer, L., Allgöwer, S., & Holzhauser, T.
  • Rapid isothermal point-of-care test for screening of SARS-CoV-2 (2023)
    Zingg, J.-M., Yang, Y.-P., Seely, S., Joshi, P., Roshid, M. H. O., Iribarren Latasa, F., O’Connor, G., Alfaro, J., Riquelme, E., Bernales, S., Dikici, E., Deo, S., & Daunert, S.
  • Rapid molecular assays for the detection of the four dengue viruses in infected mosquitoes (2022)
    Ahmed, M., Pollak, N. M., Hugo, L. E., van den Hurk, A. F., Hobson-Peters, J., Macdonald, J., & Paradkar, P. N.
  • Rapid molecular detection of CMY-2, and CTX-M group 1 and 9 variants via recombinase polymerase amplification (2023)
    Ertl, N. G., Irwin, A. D., Macdonald, J., Bauer, M. J., Wang, C. Y. T., Harris, P. N. A., Heney, C., Zowawi, H. M., & Whiley, D. M.
  • Rapid onsite detection of piper yellow mottle virus infecting black pepper by recombinase polymerase amplification-lateral flow assay (2023)
    Greeshma, M., Bhat, A. I., & Jeevalatha, A.
  • Rapid PCR-lateral flow assay for the onsite detection of Atlantic white shrimp (2023)
    Kwawukume, S., Velez, F. J., Williams, D., Cui, L., & Singh, P.
  • Rapid sample preparation and low-resource molecular detection of hepatopancreatic parvoviruses (HPV) by recombinase polymerase amplification lateral flow detection assay in shrimps (Fenneropenaeus merguiensis). (2022)
    Pollak ID, N. M., Fais, O., Kristoffersen, J., Phuthaworn, C., Knibb, W., & Macdonald, J. I.
  • Rapid SARS-CoV-2 testing in primary material based on a novel multiplex LAMP assay (2020)
    Schermer B., Fabretti F., Damagnez M., et. al.
  • Rapid Visual CRISPR Assay: A Naked-Eye Colorimetric Detection Method for Nucleic Acids Based on CRISPR/Cas12a and a Convolutional Neural Network (2022)
    Xie, S., Tao, D., Fu, Y., Xu, B., Tang, Y., Steinaa, L., Hemmink, J. D., Pan, W., Huang, X., Nie, X., Zhao, C., Ruan, J., Zhang, Y., Han, J., Fu, L., Ma, Y., Li, X., Liu, X., & Zhao, S.
  • Rapid visual detection of cyprinid herpesvirus 2 by recombinase polymerase amplification combined with a lateral flow dipstick (2018)
    Wang H, Sun M, Xu D, Podok P, Xie J, Jiang Y, Lu L
  • Rapid visual detection of Enterocytozoon hepatopenaei by recombinase polymerase amplification combined with a lateral flow dipstick (2022)
    Pang, J., Zhao, C., Liu, Z., Lu, Q., He, X., Weng, S., & Jianguo
  • Rapid visual detection of Mycobacterium avium subsp. paratuberculosis by recombinase polymerase amplification combined with a lateral flow dipstick (2018)
    Zhao G, Wang H, Hou P, He C, He H
  • Rapid Visual Detection of Pathogenic Streptococcus suis Type 2 through a Recombinase Polymerase Amplification Assay Coupled with Lateral Flow Test (2022)
    Qi, Y., Li, W., Li, X., Shen, W., Lv, R., Lu, N., Li, J., Zhuang, S., Xu, Y., Gui, Q., Shen, H., & Li, Y.
  • Rapid Visual Detection of Streptococcus suis and Actinobacillus pleuropneumoniae Through Duplex Recombinase Polymerase Amplification Combined with Lateral Flow Dipsticks (2022)
    Zhang, S., Xie, H., Liu, M., Zheng, A., Yan, H., Duan, M., Wei, X., Teng, Z., Zhang, H., & Xia, X.
  • Rapid visual nucleic acid detection of Vibrio alginolyticus by recombinase polymerase amplification combined with CRISPR/Cas13a. (2023)
    Wang, Y., Hou, Y., Liu, X., Lin, N., Dong, Y., Liu, F., Xia, W., Zhao, Y., Xing, W., Chen, J., & Chen, C.
  • Rapid, adaptable and sensitive Cas13-based COVID-19 diagnostics using ADESSO (2022)
    Casati, B., Verdi, J. P., Hempelmann, A., Kittel, M., Klaebisch, A. G., Meister, B., Welker, S., Asthana, S., Giorgio, S. di, Boskovic, P., Man, K. H., Schopp, M., Ginno, P. A., Radlwimmer, B., Stebbins, C. E., Miethke, T., Papavasiliou, F. N., & Pecori, R.
  • Rapid, CRISPR-based, field-deployable detection of white spot syndrome virus in shrimp (2019)
    Sullivan TJ, Arun KD, Cruz-Flores R, Bodnar AG
  • Rapid, Sensitive, and Specific Severe Acute Respiratory Syndrome Coronavirus 2 Detection: A Multicenter Comparison Between Standard Quantitative Reverse-Transcriptase Polymerase Chain Reaction and CRISPR-Based DETECTR (2021)
    Brandsma, E., Verhagen, H., van de Laar, T., Claas, E., Cornelissen, M., & van den Akker, E.
  • Rapid, sensitive, and specific, low-resource molecular detection of Hendra virus (2023)
    Pollak, N. M., Marsh, G. A., Olsson, M., McMillan, D., & Macdonald, J.
  • Rational Programming of Cas12a for Early-Stage Detection of COVID-19 by Lateral Flow Assay and Portable Real-Time Fluorescence Readout Facilities (2022)
    Yi, Z., de Dieu Habimana, J., Mukama, O., Li, Z., Odiwuor, N., Jing, H., Nie, C., Hu, M., Lin, Z., Wei, H., & Zeng, L.
  • Recombinase Polymerase Amplification and Lateral Flow Assay for Ultrasensitive Detection of Low-Density. (2020)
    Lalremruata A., Nguyen T., MCCall M., et. al.
  • Recombinase polymerase amplification assay combined with a dipstick-readout for rapid detection of Mycoplasma ovipneumoniae infections (2021)
    Gupta, S. K., Deng, Q., Gupta, T. B., Maclean, P., Jores, J., Heiser, A.
  • Recombinase polymerase amplification assay combined with a lateral flow dipstick for rapid detection of Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonids. Parasit Vectors. 2018 Apr 11;11(1):234. (2018)
    Soliman H. et al.
  • Recombinase polymerase amplification combined with a lateral flow dipstick for discriminating between infectious Penaeus stylirostris densovirus and virus-related sequences in shrimp genome (2014)
    Jaroenram, W., & Owens, L.
  • Recombinase Polymerase Amplification Combined with Lateral Flow Strip for Rapid Detection of OXA-48-like Carbapenemase Genes in Enterobacterales (2022)
    Hemwaranon, P., Srisrattakarn, A., Lulitanond, A., Tippayawat, P., Tavichakorntrakool, R., Wonglakorn, L., Daduang, J., & Chanawong, A.
  • Recombinase polymerase amplification lateral flow dipstick (RPA-LF) detection of Babesia orientalis in water buffalo (Bubalus babalis, Linnaeus, 1758) (2021)
    An, X., Zhao, Y., Cui, J., Liu, Q., Yu, L., Zhan, X., Zhang, W., He, L., & Zhao, J.
  • Recombinase polymerase amplification with lateral flow strip for detecting Babesia microti infections (2021)
    Nie, Z., Zhao, Y., Shu, X., Li, D., Ao, Y., Li, M., Wang, S., Cui, J., An, X., Zhan, X., He, L., Liu, Q., & Zhao, J.
  • Recombinase polymerase amplification-lateral flow (RPA-LF) assay combined with immunomagnetic separation for rapid visual detection of Vibrio parahaemolyticus in raw oysters. (2020)
    Jiang W., Ren Y., Han X., Xue J., Shan T., Chen Z., Liu Y., Quan W.
  • Recombinase Polymerase Amplification/Cas12a-Based Identification of Xanthomonas arboricola pv. pruni on Peach (2021)
    Luo, M., Meng, F. Z., Tan, Q., Yin, W. X., & Luo, C. X.
  • REVEALR: A Multicomponent XNAzyme-Based Nucleic Acid Detection System for SARS-CoV-2 (2021)
    Yang, K., & Chaput, J. C.
  • Reverse transcription loop-mediated isothermal amplification combined with lateral flow device (RT-LAMP-LFD) for swine influenza virus detection (2021)
    Asih, A. U., Janetanakit, T., Nasamran, C., Bunpapong, N., Boonyapisitsopa, S., Amonsin, A.
  • Reverse Transcription-Loop-Mediated Isothermal Amplification-CRISPR-Cas13a Technology as a Promising Diagnostic Tool for SARS-CoV-2 (2022)
    Ortiz-Cartagena, C., Fernández-García, L., Blasco, L., Pacios, O., Bleriot, I., López, M., Cantón, R., & Tomás, M.
  • RT-RPA-Cas12a-based assay facilitates the discrimination of SARS-CoV-2 variants of concern (2023)
    Tang, G., Zhang, Z., Tan, W., Long, F., Sun, J., Li, Y., Zou, S., Yang, Y., Cai, K., Li, S., Wang, Z., Liu, J., Mao, G., Ma, Y., Zhao, G.-P., Tian, Z.-G., & Zhao, W.
  • Sensitive and Easy-Read CRISPR Strip for COVID-19 Rapid Point-of-Care Testing (2021)
    Li, H., Dong, X., Wang, Y., Yang, L., Cai, K., Zhang, X., Kou, Z., He, L., Sun, S., Li, T., Nie, Y., Li, X., & Sun, Y.
  • Sensitive and rapid detection of Babesia species in dogs by recombinase polymerase amplification with lateral flow dipstick (RPA-LFD) (2022)
    Onchan, W., Ritbamrung, O., Changtor, P., Pradit, W., Chomdej, S., Nganvongpanit, K., Siengdee, P., Suyasunanont, U., & Buddhachat, K
  • Sensitive and Rapid Detection of Citrus Scab Using an RPA-CRISPR/Cas12a System Combined with a Lateral Flow Assay (2021)
    Shin, K., Kwon, S. H., Lee, S. C., & Moon, Y. E.
  • Sensitive and Specific Exonuclease III-Assisted Recombinase-Aided Amplification Colorimetric Assay for Rapid Detection of Nucleic Acids (2023)
    Zhou, Y., Zhang, J., Sun, H., Tao, D., Xu, B., Han, X., Ren, R., Ruan, J., Steinaa, L., Hemmink, J. D., Han, J., Li, X., Xu, J., Zhao, S., Xie, S., & Zhao, C.
  • Sensitive naked-eye detection of telomerase activity based on exponential amplification reaction and lateral flow assay (2022)
    Cheng, X. R., Wang, F., Liu, C. yun, Li, J., Shan, C., Wang, K., Wang, Y., Li, P. F., & Li, X. M
  • Sequence-Specific Detection of ORF1a, BRAF, and ompW DNA Sequences with Loop Mediated Isothermal Amplification on Lateral Flow Immunoassay Strips Enabled by Molecular Beacons (2021)
    Varona, M., Eitzmann, D. R., & Anderson, J. L.
  • SHERLOCK: nucleic acid detection with CRISPR nucleases (2019)
    Kellner MJ, Koob JG, Gootenberg JS, Abudayyeh OO, Zhang F
  • SHERLOCK4HAT: A CRISPR-based tool kit for diagnosis of Human African Trypanosomiasis (2022)
    Sima, N., Dujeancourt-Henry, A., Perlaza, B. L., Ungeheuer, M.-N., Rotureau, B., & Glover, L.
  • Simplified Cas13-based assays for the fast identification of SARS-CoV-2 and its variants (2022)
    Arizti-Sanz, J., Bradley, A., Zhang, Y. B., Boehm, C. K., Freije, C. A., Grunberg, M. E., Kosoko-Thoroddsen, T.-S. F., Welch, N. L., Pillai, P. P., Mantena, S., Kim, G., Uwanibe, J. N., John, O. G., Eromon, P. E., Kocher, G., Gross, R., Lee, J. S., Hensley, L. E., MacInnis, B. L., Myhrvold, C.
  • Specific Detection of Influenza A and B Viruses by CRISPR-Cas12a-Based Assay (2021)
    Park, B. J., Park, M. S., Lee, J. M., & Song, Y. J.
  • Supporting Information Clustered Regularly Interspaced Short Palindromic Repeats / Cas9- Mediated Lateral Flow Nucleic Acid Assay. (2020)
    Wang X., Xion E., Tian T., Cheng M., Lin W., Wang H.
  • The development of highly specific and sensitive primers for the detection of potentially allergenic soybean (Glycine max) using loop-mediated isothermal amplification combined with lateral flow dipstick (LAMP-LFD). (2020)
    Allgöwer S., Hartmann C., Holzhauser T.
  • The Development of Loop-Mediated Isothermal Amplification Combined with Lateral Flow Dipstick for Detection of Karlodinium veneficum. Harmful Algae 2017; 62, 20-29. (2017)
    Huang HL et al.
  • Time course of detection of human male DNA from stained blood sample on various surfaces by Loop Mediated Isothermal Amplification and Polymerase Chain Reaction (2018)
    Kanchanaphum P
  • Toward a CRISPR-Based Point-of-Care Test for Tomato Brown Rugose Fruit Virus Detection (2022)
    Bernabé-Orts, J. M., Hernando, Y., & Aranda, M. A.
  • Trends and Innovations in Biosensors for COVID-19 Mass Testing (2020)
    Santiago I.
  • Ultrasensitive ImmunoMag-CRISPR Lateral Flow Assay for Point-of-Care Testing of Urinary Biomarkers (2023)
    Lee, I., Kwon, S.-J., Heeger, P., & Dordick, J. S.
  • Use of a recombinase polymerase amplification commercial kit for rapid visual detection of Pasteurella multocida (2019)
    Zhao G, He H, Wang H
  • Use of reverse transcription loop-mediated isothermal amplification combined with lateral flow dipstick for an easy and rapid detection of Jembrana disease virus. Virusdisease. 2015 Sep; 26(3):189-95 (2015)
    Kusumawati A. et al.
  • Utilization of recombinase polymerase amplification combined with a lateral flow strip for detection of Perkinsus beihaiensis in the oyster Crassostrea hongkongensis (2019)
    Wu L, Ye L, Wang Z, Cui Y, Wang J
  • Variety Differentiation: Development of a CRISPR DETECTR Method for the Detection of Single Nucleotide Polymorphisms (SNPs) in Cacao (Theobroma cacao) and Almonds (Prunus dulcis) (2023)
    Wax, N., La-Rostami, F., Albert, C., & Fischer, M.
  • Vigilant: An Engineered VirD2-Cas9 Complex for Lateral Flow Assay-Based Detection of SARS-CoV2 (2021)
    Marsic, T., Ali, Z., Tehseen, M., Mahas, A., Hamdan, S., & Mahfouz, M.
  • Visual Detection of Canine Parvovirus Based on Loop-Mediated Isothermal Amplification Combined with Enzyme-Linked Immunosorbent Assay and with Lateral Flow Dipstick. J. Vet. Med. Sci. 2014 (2014)
    Sun YL et al.
  • Visual detection of porcine reproductive and respiratory syndrome virus using CRISPR-Cas13a. (2020)
    Chang Y., Deng Y., Li T.
Videos

FAQs
A:

Yes, but initially negative strips may a faint band after prolonged time, while positive bands will not disappear! We recommend to take an image of the strip shortly after the incubation time is over.

A:

HybriDetect allows the detection of 1 target on the strip, while HybriDetect 2T allows the detection of 2 targets on the same strip.

A:

This depends on the size of the amplicon. The shorter the amplicon is, the lower is the sensitivity of the agarose gel. If the amplicon is 100 bases long HybriDetect could possibly be 1.000 fold more sensitive compared to the agarose gel.

A:

Yes, but initially negative strips may a faint band after prolonged time, while positive bands will not disappear! We recommend to take an image of the strip shortly after the incubation time is over.

A:

Yes, we accept credit card for payment if you purchase our products online via our webshop.

A:

Yes, you can download the pdf-file of the MSDS for HybriDetect here here and for HybriDetect 2T here.

A:

The products are in inventory and we ship the same day if we receive the order prior 12.00 CET.

A:

No, kits can be shipped at ambient temperatures, but should be stored at 2 – 8°C after receipt.

A:

Yes, the kits will still perform well. We have made stability and could demonstrate that the kits still perform well after storing them for 100 days at 37°C.

A:

The sum of the volume of the sample and the buffer should not exceed 100 µl.

A:

This phenomenon is due to the formation of primer dimers. In this event biotinylated and FITC-labeled primers bind to each other and form dimers resulting in false positive results. You need to redesign your primers in order to prevent primer dimerization.

A:

The shelf life of the kits from the time of production is 24 months. Milenia Biotec is manufacturing fresh lots twice a year, meaning that our customers usually get kits with a remaining shelf life of not less than 12 months.

A:

Yes, in case the concentration of labeled primers or probes is too high, this may lead to a decreased sensitivity of the test. In this case primer or probe concentration need to be reduced. Furthermore, components of the sample or the sample buffer, including SDS, DTT or urea may have a negative impact on the performance of the test. In this case the sample can be diluted in order to „dilute the effect out“. However this approach will result in a reduced sensitivity of the test. It is also possible to develop a modified assay buffer to reduce the interferences.

A:

Yes, we demonstrated that HybriDetect (MGHD 1) still performs well at temperatures up to 75°C and HybriDetect 2T at temperatures up to 65°C.

290
100

Usted podría estar interesado también en los siguientes productos:



Referencia
Descripción
Cond.
Precio Sin IVA
MGHD21
Milenia® GenLine HybriDetect 2T
 100tests