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Cycler
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Cycler

Cycler

PCR cycler compatibility

  • Specification

    Product Description

    miRNA Screening Panel enables the sample and standardized analysis of specific circulating microRNAs in human serum and plasma, which reflects bone quality.

    Supplied Product

    Kit content:

    A. Serum/Plasma RNA extraction
    1. Lysis Buffer
    2. Protein Precipitation Buffer
    3. Wash Buffer 1
    4. Wash Buffer 2
    5. Rnase-free water
    6. Spin columns
    7. Collection tube 1.5 mL
    8. Collection tube 2 mL

    B. cDNA synthesis
    1. RNA spike-in Mix
    2. cDNA spike-in Mix
    2. 5x RT Buffer
    3. 10x RT Enzyme Mix
    4. RNase-free water
    5. 2x miGreen Mix
    6. Glycogen (5mg/mL)

    C. qPCR
    1. Primer coated 384 well qPCR plates

    Pack Size

    384 reactions

    Suitable Sample

    Serum, Plasma

    Sample Size

    200 μL of human serum

    Regulation Status

    For research use only (RUO)

    Quality Control Testing

    Screening

    Storage Instruction

    Store the kit at -20°C.

    Note

    Cycler

  • Applications

    Screening

    Summary of evidence for miRNA bone biomarkers included in the kit.