Mitapivat [1260075-17-9]
Referencia HY-12689-5mg
embalaje : 5mg
Marca : MedChemExpress
Mitapivat (AG-348) is an orally active pyruvate kinase allosteric activator. Mitapivat increases enzymatic activity, protein stability, and ATP levels over a broad range of PKLR genotypes, shows the potential to restore the activity of PK (pyruvate kinase)-deficient glycolytic pathways. Mitapivat can be used in study of PK deficiency.
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Mitapivat Estructura química
No. CAS : 1260075-17-9
This product is a controlled substance and not for sale in your territory.
Descripciòn |
Mitapivat (AG-348) is an orally active pyruvate kinase allosteric activator. Mitapivat increases enzymatic activity, protein stability, and ATP levels over a broad range of PKLR genotypes, shows the potential to restore the activity of PK (pyruvate kinase)-deficient glycolytic pathways. Mitapivat can be used in study of PK deficiency[1][2][3]. |
IC50 & Target |
pyruvate kinase[1][2][3]. |
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In Vitro |
Mitapivat (0.1 nM-100 μM; 16 h) activates WT PK-R in RBCs from healthy donors[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability Assay[1]
Cell Viability Assay[1]
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In Vivo |
Mitapivat (50 mg/kg; p.o.; twice daily for 21 days) improves anemia in a mouse model for β-thalassemia[2]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Ensayo clínico |
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Peso molecular |
450.55 |
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Fòrmula |
C24H26N4O3S |
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No. CAS |
1260075-17-9 |
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Appearance |
Solid |
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Color |
White to off-white |
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SMILES |
O=S(C1=C2N=CC=CC2=CC=C1)(NC3=CC=C(C(N4CCN(CC5CC5)CC4)=O)C=C3)=O |
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Envío | Room temperature in continental US; may vary elsewhere. |
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Almacenamiento |
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Solvente y solubilidad |
In Vitro:
DMSO : 20.83 mg/mL (46.23 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Pureza y Documentación |
Purity: 99.83% |
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Referencias |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 2.2195 mL | 11.0975 mL | 22.1951 mL | 55.4877 mL |
5 mM | 0.4439 mL | 2.2195 mL | 4.4390 mL | 11.0975 mL | |
10 mM | 0.2220 mL | 1.1098 mL | 2.2195 mL | 5.5488 mL | |
15 mM | 0.1480 mL | 0.7398 mL | 1.4797 mL | 3.6992 mL | |
20 mM | 0.1110 mL | 0.5549 mL | 1.1098 mL | 2.7744 mL | |
25 mM | 0.0888 mL | 0.4439 mL | 0.8878 mL | 2.2195 mL | |
30 mM | 0.0740 mL | 0.3699 mL | 0.7398 mL | 1.8496 mL | |
40 mM | 0.0555 mL | 0.2774 mL | 0.5549 mL | 1.3872 mL |