Mouse Anti MHC Class I (H-2K)

Mouse Antibody Monoclonal.

MHC Class I is a membrane spanning molecule consisting of 2 proteins. MHC Class I is just about 350 amino acids in length, with roughly 75 amino acids at the carboxylic end comprising the transmembrane and cytoplasmic portions. The remaining 270 amino acids, are divided into three globular domains labeled Alpha-1, Alpha-2 and Alpha-3 prime, with Alpha-1 being closest to the amino terminus and Alpha-3 closest to the membrane. The second portion of the molecule is a small globular protein called Beta-2 Microglobulin It links primarily with the Alpha-3 prime domain and is essential for MHC stability.
The MHC ability to show a vast range of antigenic peptides for T cell recognition needs a compromise between broad specificity and high affinity. Both MHC class I and MHC class II show an exceptional structure while the peptide main chain is tightly bound and peptide side chains show less restrictive interactions. It is primarily the peptide side-chain contacts and conformational variability that ensures that the peptide-MHC complex presents an antigenically unique surface to T cell receptors.

NYRmH-2K.

Purified mouse LN cells (C57Bl/6 anti-BALB/c).

Mouse IgG2b.

Recognizes most mouse H-2K haplotypes (k,p,q,r,s). Does NOT react with H-2kd.

Ion exchange column.

1mg/ml in PBS (after reconstitution).

Lyophilized: store at 4°C. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.

Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Cytotoxic and staining antibody. For staining, use 10ml/1,000,000 cells. Titer for cytotoxicity should be determined by the investigator.

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